This
study describes a technical platform that allows us to measure the
pore-forming activity of antimicrobial peptides (AMPs) in the lipid
bilayer and estimate antimicrobial activity. We selected six different
AMPs of diverse species from urochordata to vertebrata and measured
the channel current signals using a microfabricated lipid bilayer
system. As a result of the electrophysiological measurements, we were
able to estimate the pore-forming activity and roughly predict the
antimicrobial activity although there was not a strong correlation
between the pore-forming activity and the variety of species. Our
method will be a unique tool for analyzing a wide variety of diverse
AMPs.
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