A eDNA clone coding for rice elongation factor Ifl' (EF-I~') was isolated from a rice anther eDNA library. The clone, named RB', was 980 bp long and contained a single open reading frame coding for 223 amino acids; the first 31 amino acids, except for the first methionine, which is absent in the mature protein, are identical to those of the purified protein determined with a protein sequencer. The amino acid sequence of rice EF-Iff shows homology to the C-terminal half of Artemia salina EF-Ifl (59%) and human EF-I//(63%), but might not have a phosphorylation site for casein kinase II which has been conserved in Artemla sallna EF-Ifl and EF-I& human EF-Ifl and silkworm EF-Ifl'. from rice. We show here the first plant eDNA sequence encoding an EF-Ifl' which does not have the conserved phosphorylation site.

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