Cleome gynandra is a widespread medicinal plant belonging to the family Capparaceae. In Ayurvedic medicine C. gynandra is a main component in Narayana Churna. It has numerous properties like Anthelmintic, in ear diseases, pruritis and several other diseases like gastro intestinal disorders and gastrointestinal infections etc. This is an effort to gather and document evidence on different features of C. gynnadra and highlight the need for survey and development. In this current study, nine proteins of C. gynandra were identify by using of bioinformatics tools. The bioinformatic study of the characterization of proteins of C.gynandra were using Expasy Protparam server, 3D structure was done using SWISS MODEL. Plants ofdifferent family show uniqueness 98% and above were particular and its sequences retrieved, aligned using Clustal Omega. Secondary Structure prediction exhibited that α – helix, random coil, β – turn and long strand leads. Phylogenetic analysis of Glyceraldehyde 3 PO4 of C. gynandra exposes that the Capparaceae families are closely related. Insilco sequence analysis of C. gynandra showed that these proteins taken from different organisms linked organized evolutionarily as they possess conserved regions in their protein sequences.These results will be helpful to further study on C. gynandra protein functions at molecular or structural levels and also valuable in homology modelling and insilico approach.

Free-radical reactions have been implicated in the pathology of many human diseases like atherosclerosis, ischemic heart disease, aging process, inflammation, diabetes, immuno-suppression,neurodegenerative disease etc. Radicals and other reactive oxygen species are formed constantly in the human body and are removed by the enzymatic and non-enzymatic antioxidant defence. The disturbance in ‘redox homeostasis’ that occurs when antioxidant defences are inadequate can damage lipids, proteins,carbohydrates and DNA. Drugs with multiple protective mechanisms, including antioxidant activity, may be one way of minimizing tissue injury. Phytochemicals with antioxidant property are naturally present in food are of great interest due to their beneficial effects on human health as they offer protection against oxidative deterioration. Amygdalin, also known as vitamin B17 is a cyanogenic glycoside found in several sources mainly in apples, pears, apricots, plums, peaches. Several reports claim amygdalin to be good chemopreventive agent, however these claims are not often backed by proper scientific evidence. Thus the present study is aimed to evaluate the therapeutic potential of amygdalin isolated from Prunus dulcis by studying its in vitro antioxidant and cytotoxic properties.Free-radical reactions have been implicated in the pathology of many human diseases like atherosclerosis, ischemic heart disease, aging process, inflammation, diabetes, immuno-suppression,neurodegenerative disease etc. Radicals and other reactive oxygen species are formed constantly in the human body and are removed by the enzymatic and non-enzymatic antioxidant defence. The disturbance in ‘redox homeostasis’ that occurs when antioxidant defences are inadequate can damage lipids, proteins,carbohydrates and DNA. Drugs with multiple protective mechanisms, including antioxidant activity, may be one way of minimizing tissue injury. Phytochemicals with antioxidant property are naturally present in food are of great interest due to their beneficial effects on human health as they offer protection against oxidative deterioration. Amygdalin, also known as vitamin B17 is a cyanogenic glycoside found in several sources mainly in apples, pears, apricots, plums, peaches. Several reports claim amygdalin to be good chemopreventive agent, however these claims are not often backed by proper scientific evidence. Thus the present study is aimed to evaluate the therapeutic potential of amygdalin isolated from Prunus dulcis by studying its in vitro antioxidant and cytotoxic properties.