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(—)‐trans‐Δ9‐Tetrahydrocannabinol (Δ9‐THC) was isolated from marihuana plant extract, by adsorptive column and glc. The adsorptive column chromatography method consisted of chromatographing marihuana extract on a column packed with a mixture of silica gel (gas chromatography grade (100/120 mesh), silver nitrate and calcium sulphate (CaSO4·H2O) (3:1:0·5) with benzene as the eluting solvent. The glc method consisted of chromatographing the extract on a 3 ft silanized glass column (3/8 inch o.d.) packed with 1·5 ft of 2% QF‐1 and 1·5 ft of 2% OV‐17 on chromosorb W, AW 30–60 mesh, prep grade. A purity of 99% for the isolated Δ9‐THC was confirmed by infrared spectroscopy, nuclear magnetic resonance, mass spectroscopy. The effects of storage conditions on Δ9‐THC stability, monitored by glc, indicated the best method for preserving Δ9‐THC was at 0°, protected from light, stored under nitrogen.