Background: Treating neurological disorders is still a major challenge, especially diseases like alzheimer's. Dragea volubilis is a wonder herb with many pharmacological activities like neuroprotective, antioxidant, anti-tumor, and anti-inflammatory. Dragea volubilis is synonymously known as Wattakaka volubilis. Chemical exploration of Dragea volubilis has yielded polyoxypregnane glycosides. Objectives: This study focused to explore the cognition-enhancing and neuroprotective functions of chloroform extract of Dragea volubilis in young and old mice in interoceptive and exteroceptive models. Materials and Methods: The chloroform extract of Dragea volubilis (CDV) was prepared by the soxhlation method. The antioxidant, anticholinesterase, and behavioral parameters were assessed through avoidance, elevated plus, and morris water maze. In-vivo studies were conducted by dividing the animals into 4 groups exteroceptive and 5 interoceptive(scopolamine-induced amnesia) 6 animals in each group. The scopolamine was administered at 2mg/kg and the extract of Dragea volubilis was administered at two different doses (100, and 200 mg/kg) for 14 days. Animals were sacrificed after behavioral parameters and brains were isolated for antioxidants and acetylcholinesterase assays. Results: A marked increase in step-down latency (SDL-passive avoidance) and a significant reduction in transfer latency (TL) in the elevated plus maze were noticed with the extract. It also demonstrated significant improvement in memory in the morris water maze (MWM) test in both the training and retention trials. CDV inhibited acetylcholinesterase and reduced thiobarbituric acid reactive substance activity (TBARS) and increased glutathione (GSH) and catalase in the mice brain showing significant antioxidant properties. Conclusion: It can be concluded with results that CDV has significant potential as a nootropic and antioxidant with neuroprotective properties.

The present study aims to prepare a methanolic extract of Dragea volubilis and to quantitatively estimate kaempferol and chlorogenic acid using newly developed and validated method of high-performance thin layer chromatography (HPTLC) using TLC densitometry technique. Dried leaves obtained from D. volubilis were defatted with petroleum ether and extracted in a Soxhlet extractor with methanol. The solvent was evaporated using a Rota evaporator. Quantitative analysis of the extract was done using TLC and HPTLC. A mixture of toluene: ethyl acetate: glacial acetic acid (5.5:4:0.5) for kaempferol and ethyl acetate: acetic acid: water (7:1.5:1.5) for chlorogenic acid was used as a mobile phase. The chromatography was performed on a TLC plate precoated with silica gel GF254 and the developed TLC plates were visualized and quantified at 367 nm and 327 nm for kaempferol and chlorogenic acid, respectively. The percentage yield was found to be 0.0397 ± 0.0001 and 0.0755 ± 0.0000 for kaempferol and 0.0755 ± 0.0000 for chlorogenic acid. Rf. values 0.95 and 0.61 were observed for kaempferol and chlorogenic acid, respectively. The method was validated for instrumental precision, repeatability, coefficient of determination (r2), linearity range (ng), LOD (ng), LOQ (ng), and intra-day and inter-day precision for both kaempferol and chlorogenic acid, and all the parameters were found to be within the acceptable range as per the ICH guidelines. The newly developed HPTLC method was found to be prompt, cost-effective, precisely accurate, and reproducible for the qualitative as well as quantitative analysis of kaempferol and chlorogenic acid in methanolic extract of D. volubilis.