Background: Platychaete aucheri is an endemic plant to the south of Iran and no phyochemical investigations is reported on the seeds oil. Materials and Methods: The chemical composition of Platychaete aucheri seeds oil was extracted by suxhelet apparatus, methyl esterificated and analyzed by GC and GC/MS spectroscopy method. Results: 16 compounds were determined in total and taraxasterol (19.0%), γ-sitosterol (14.1%) and lupeol (11.8%) were characterized as the most abundant components. Conclusion: Regarding the presence of high amounts of triterpenoids and sterols in the seed oil, related biological activities are expected from the studied oil.