Objective: Assessment of the macular ganglion cells function in myopic subjects using pattern electroretinogram (PERG) in comparison with healthy controls to help in diagnosing of retinal diseases, and studying the relationship of the changes seen in PERG with the axial length (AL) of the eye. Methods: The study was cross sectional, included 62 subjects (121 eyes). Three groups were examined: group 1: controls or emmetropics; group 2: mild myopics; group 3: moderate myopics. For all participants, a detailed pathological story was taken, a comprehensive eye examination was performed, AL was measured, and PERG was recorded, then the variables of the recorded waves (peak time and amplitude) were analyzed. Results: A statistically significant decrease in mean amplitudes of (P50, N95) waves was observed in group 2 (36 eyes) and 3 (25 eyes) compared to group 1 (60 eyes) (P-value <0.01), and a statistically significant elongation of mean N95 peak time was observed in (group 2 and 3) compared to group 1 (P-value <0.01) without being associated with elongation of mean P50 peak time. By studying the relationship between AL and each of peak time and amplitude of (P50, N95) waves, we have found that when AL increased, amplitudes decreased and peak times prolonged in a statistically significant way (P-value <0.01). Conclusion:The decreased amplitude of P50 wave may indicate a functional impairment of the macula in myopic subjects, and the decreased amplitude of N95 wave may be due to a functional impairment of their ganglion cells. The elongation of P50 peak time with increasing of AL may be due to a delay of the synaptic transmission between photoreceptors, bipolar cells, and ganglion cells.