Ice-ice disease in cultivated algae occurs due to pathogenic bacterial infections. Generally, ice-ice disease is characterized by whitening of the branches and initiated with the color changes of the thalli becomes transparent. This study was aimed to isolate and identify bacteria on seaweed Gracilaria verrucosa infected by ice-ice. Isolated bacteria was inoculated in Tryptic Soy Agar (TSA) and Thiosulphate Citrate Bile Sucrose (TCBS). Morphological and biochemical characterization of the bacterial isolates revealed eight species of bacteria were found in infected thalli including Acinetobacter sp., Pseudomonas sp., Bacillus sp., Micrococcus sp., Corinoverm sp., Cytophaga sp., Vibrio mimicus and V. Campbelii, but not all identified bacteria are pathogens on Gracilaria verrucosa. The pathogenic bacteria were Acinetobacter sp, Pseudomonas sp, Bacillus sp, Cytophaga sp. and Vibrio sp.
Rumput laut jenis Eucheuma cottonii adalah salah satu hasil perikanan yang mempunyai nilai ekonomis cukup penting karena mengandung karagenan yang merupakan bahan dasar dalam industri farmasi, makanan, maupun bahan industri lain. Mengingat besarnya permintaan pasar akan kebutuhan rumput laut maka perlu dilakukan usaha untuk meningkatkan produksinya. Namun seiring dengan meningkatnya permintaan, pembudidaya sering mengalami permasalahan antara lain bibit rumput laut yang dihasilkan belum maskimal baik dari segi jumlah maupun kualitas, sehingga perlu ada kajian untuk menghasilkan pertumbuhan benih bibit rumput laut yang baik yaitu menggunakan pupuk Conway pada media kultur rumput laut dari hasil kultur jaringan. Pupuk conway mengandung unsur nutrien yang sangat bermanfaat bagi pertumbuhan rumput laut. Penelitian ini bertujuan untuk mendapatkan dosis pupuk Conway yang tepat pada media kultur terhadap pertumbuhan bibit rumput laut E. cottonii. Penelitian ini menggunakan E.cottoni dari hasil kultur jaringan Seameo Biotrop. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 5 ulangan yaitu dosis pupuk Conway (0, 2, 4, 6, dan 8 ml). Padat tebar E.cottoni yaitu 5 eksplan per wadah. Hasil penelitian didapatkan bahwa pemberian pupuk Conway dengan dosis berbeda berpengaruh sangat nyata (P<0.01) terhadap pertumbuhan panjang mutlak dan laju pertumbuhan spesifik, serta pertumbuhan bobot mutlak E. cottonii, dengan nilai tertinggi yaitu masing-masing 1,04 cm, 0,014%, dan 0,25 g pada perlakuan dosis pupuk Conway 8 ml. Seaweed Eucheuma cottonii is one of the fishery products that have highly important economic value because it contains carrageenan which is a basic ingredient in pharmaceuticals, food, and other industrial materials. Given the large market demand for seaweed, it is necessary to make efforts to increase production. However, along with increasing demand, cultivators often experience problems, the constraints that are often experienced are, among others, the seaweed seeds produced are not optimal both in terms of quantity and quality, so there needs to be a study to produce good seaweed seed growth, namely using Conway fertilizer on seaweed culture media from tissue culture results. Conway fertilizer contains nutrients that are very beneficial for the growth of seaweed. This study aims to obtain the correct dosage of Conway fertilizer in culture media on the growth of E. cottonii seaweed seedlings. This study used E.cottoni from the Seameo Biotrop tissue culture. It used a completely randomized design with 5 treatments and 5 replications, namely the dose of Conway fertilizer (0, 2, 4, 6, and 8 ml). The stocking density of E. cottoni was 5 explants per container. The results showed that the application of Conway fertilizer with different doses had a very significant effect (P<0.01) on absolute length growth, specific growth rate, and absolute weight growth of E. cottonii, with the highest values of 1.04 cm, 0.014%, and 0,25 g, respectively, at the Conway fertilizer dose of 8 mL.
One of the causes of the low production of koi carp seeds is fungal attack during the egg-hatching period, so natural treatments need to be carried out, including the use of papaya seeds. Papaya seeds contain antifungal compounds in the form of tannins, flavonoids, saponins, and alkaloids. The purpose of this study was to determine the effective dose of papaya seed solution in reducing the prevalence of the fungus and increasing the hatchability of koi goldfish eggs. This study was designed using a completely randomized design, which consisted of 5 treatments with 4 replications each. The treatment tested was papaya seed solution with treatment doses A=control, B=125 mg L-1, C=150 mg L-1, D=175 mgL-1, and E=200 mg L-1. Variables measured included the prevalence of the fungus, egg hatchability, observation of the fungus, hatching time, and water quality. The results showed that treatment with 125 mg L-1 of papaya seed solution reduced the prevalence of the fungus by 32.5% and resulted in the highest egg hatchability of 67.5%. The high hatchability of eggs is due to the presence of antifungal compounds in the form of flavonoids, saponins, tannins, and karpain contained in papaya seeds which provide protection for eggs against fungal infections so that eggs can hatch properly. The best dose of papaya seed solution to prevent fungal infection in koi carp eggs obtained at 200 mg L-1, 11.25%.
South Banawa District is one of the areas in Donggala Regency which has the best prospects in the pond aquaculture sector because this area has the potential to produce milkfish and shrimp. The earthquake and tsunami of 28 September 2018 damaged most of the ponds in South Banawa District. This study aimed to analyze the composition, diversity, and dominance of plankton in fish and shrimp farming ponds in Lalombi and Tolongano Village. The diversity index (H ́) of plankton in the two villages, namely Lalombi, ranges from 0.3040-1.0769 (phytoplankton), 0.4385-0.4610 (zooplankton) and Tolongano, it ranges from 0.2219-1.2318 (phytoplankton), 0-1.3297 (zooplankton). The results of this diversity are included in the low community category, which means that the condition of the plankton community is unstable, so the condition of the community is experiencing environmental disturbances, caused by changes in the structure of the soil texture due to the earthquake and tsunami. The dominance index values ranged from 0.4227-0.9033 for and from 0-0.2778 for Dominance index values close to one mean that under current conditions there is a dominant type of but for the value was close to zero, which means that there was no species dominating the structure of the community. uniformity index values ranged from 0.1562-0.4957 and those for ranged from 0-0.9592.
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