A series of 3,3-arylidene bis (4-hydroxycoumarins) 2 were synthesized by the reaction of aromatic aldehydes with
4-hydroxycoumarin
using dodecylbenzenesulfonic acid as Brønsted acid-surfactant
catalyst in aqueous media and under microwave irradiation. The present
method is operationally simple and the use of water as the reaction
medium makes the process environmentally benign. The epoxydicoumarins 5 were then obtained with a good yield by heating 3,3′-arylidenebis-4-hydroxycoumarins 2 in acetic anhydride. Techniques such as elemental analysis, 1H, 13C–1H NMR, and infrared spectroscopy
were employed to characterize these compounds. The synthesized compounds
displayed good antibacterial potential against Escherichia
coli (ATCC 25988), Pseudomonas aeruginosa (ATCC 27853), Klebsilla pneumonia (ATCC 700603), Staphylococcus aureus (ATCC 29213), methicillin-resistant Staphylococcus
aureus (ATCC 43300) and Candida albicans (ATCC 14053). The MIC values of 23 mg/mL for compound 5e against Escherichia coli (ATCC 25988)
and 17 mg/mL for 2a were observed. Furthemore, a molecular
docking simulation has been performed to evaluate the antibacterial
activities and the probable binding modes of the studied compounds 2a–f and 5a–g toward the active
sites of a series of well known antibacterial targets. Among the investigated
compounds, the binding modes and docking scores demonstrate that 2a has the most antibacterial and antifungal activities. Additionally,
DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS has been tested for
their ability to scavenge hydrogen peroxide and free radicals. According
to our results, these compounds exhibit excellent radical scavenging
properties. Furthermore, compounds 2–5 were evaluated
for anti-inflammatory activity by indirect haemolytic and lipoxygenase
inhibition assays and revealed good activity.
The aim of this study was devoted into molecular docking calculations to discover the potential antileishmania and antitoxoplasma activities of newly synthesized compounds obtained by applying a practical and simple method under microwave irradiation. All these compounds were tested in vitro for their biological activity against Leishmania major promastigotes, amastigotes, and Toxoplasma gondii tachyzoites. Compounds 2a, 5a, and 5e were the most active against both L. major promastigotes and amastigotes, with IC 50 values of less than 0.4 μM mL −1 . Compounds 2c, 2e, 2h, and 5d had a strong antitoxoplasma activity of less than 2.1 μM mL −1 against T. gondii. We can conclude that aromatic methyleneisoindolinones are potently active against both L. major and T. gondii. Further studies for mode of action evaluation are recommended. Compounds 5c and 5b are the best drug candidates for antileishmania and antitoxoplasma due to their SI values being over 13. The docking studies of compounds 2a-h and 5a-e against pteridine reductase 1 and T. gondii enoyl acyl carrier protein reductase reveal that compound 5e may be an effective antileishmanial and antitoxoplasma drug discovery initiative.
In the present work, a combination of experimental and density functional theory(DFT) investigation of the (3+2) cycloaddition reactions of diazopropane with chalcone derivatives was reported. All calculations were performed using several DFT approaches (B3LYP, M06, M06-2X) and 6-311+G(d, p) basis set. Based on the NMR, MS analyses and IRC calculations, the pyrazole derivatives are the kinetic adducts over the oxadiazoles. The use of two equivalents of diazopropane leads to thermodynamical products. A molecular docking analysis was performed to investigate the efficiency of the obtained products against selected drug targets in anti-Alzheimer ligand-receptor interactions. We revealed that the ligands selected were bound mainly to the catalytic (CAS) and peripheral (PAS) anionic sites of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitors, respectively. The selected ligands 1, 3, 4 and P14 may act as the best inhibitors against Alzheimer’s disease (AD).
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