The discovery of semiconducting behavior of deoxyribonucleic acid (DNA) has resulted in a large number of literatures in the study of DNA electronics. Sequence-specific electronic response provides a platform towards understanding charge transfer mechanism and therefore the electronic properties of DNA. It is possible to utilize these characteristic properties to identify/detect DNA. In this current work, we demonstrate a novel method of DNA-based identification of basidiomycetes using current-voltage (I-V) profiles obtained from DNA-specific Schottky barrier diodes. Electronic properties such as ideality factor, barrier height, shunt resistance, series resistance, turn-on voltage, knee-voltage, breakdown voltage and breakdown current were calculated and used to quantify the identification process as compared to morphological and molecular characterization techniques. The use of these techniques is necessary in order to study biodiversity, but sometimes it can be misleading and unreliable and is not sufficiently useful for the identification of fungi genera. Many of these methods have failed when it comes to identification of closely related species of certain genus like Pleurotus. Our electronics profiles, both in the negative and positive bias regions were however found to be highly characteristic according to the base-pair sequences. We believe that this simple, low-cost and practical method could be useful towards identifying and detecting DNA in biotechnology and pathology.
The exciting discovery of the semiconducting-like properties of deoxyribonucleic acid (DNA) and its potential applications in molecular genetics and diagnostics in recent times has resulted in a paradigm shift in biophysics research. Recent studies in our laboratory provide a platform towards detecting charge transfer mechanism and understanding the electronic properties of DNA based on the sequence-specific electronic response, which can be applied as an alternative to identify or detect DNA. In this study, we demonstrate a novel method for identification of DNA from different shrimp viruses and bacteria using electronic properties of DNA obtained from both negative and positive bias regions in current-voltage (I–V) profiles. Characteristic electronic properties were calculated and used for quantification and further understanding in the identification process. Aquaculture in shrimp industry is a fast-growing food sector throughout the world. However, shrimp culture in many Asian countries faced a huge economic loss due to disease outbreaks. Scientists have been using specific established methods for detecting shrimp infection, but those methods do have their significant drawbacks due to many inherent factors. As such, we believe that this simple, rapid, sensitive and cost-effective tool can be used for detection and identification of DNA from different shrimp viruses and bacteria.
Digital agriculture involving different tools and management practices has advanced considerably in recent years, intending to overcome climate risk and reduce food insecurity. Climate change and its impacts on agricultural production and food security are significant sources of public concern worldwide. The objective of this study was to provide an overview of the potential impact of digital agriculture technologies and practices that can reduce greenhouse gas emissions and enhance productivity while ensuring food security. Based on a comprehensive survey of the previously published works, it was found that due to global warming, altered precipitation patterns, and an increase in the frequency of extreme events, climate change has negatively impacted food security by reducing agricultural yields, slowing animal growth rates, and decreasing livestock productivity. The reviewed works also suggest that using digital technology in agriculture is necessary to mitigate the effect of climate change and food insecurity. In addition, issues regarding creating sustainable agricultural food systems, minimizing environmental pollution, increasing yields, providing fair and equitable food distribution, and reducing malnutrition leading to food security were discussed in detail. It was shown that while digital agriculture has a crucial role in mitigating climate change and ensuring food security, it requires a concerted effort from policymakers, researchers, and farmers to ensure that the benefits of digitalization are realized in a sustainable and equitable manner.
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