SUMMARYWe studied the possibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bio-nanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro-and nano-dimentions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, affect of preforming protein corona on ND and F, was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the metabolic activity of cells. Therefore, in the present study the biological activity of nanomaterials was evaluated by assessing cellular reduction of MTT, so as by determining changes in cell morphology in the direct contact with samples. BNM have been shown to affect; in a size, chemical composition and morphological characteristicsdependent manner, cellular reduction of MTT, so as cell morphology: ND and F exhibited specific effects in a dose-dependent manner, which was sensitive to the formation of protein corona on the surface of the nanoparticles. Our results suggest that short-term culture of monocytes is a sensitive model system for assessing the biological effects of nanomaterials in vitro.

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The morphological and functional features of the monocytes in a short-term in vitro cultivation