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During the study of the effect of nanoparticles researches aimed at finding out the consequences of their influence on the structural and functional integrity of germ cells, which ensure genetic continuity must occupy the one of the central places. However, in the assessment of the impact of nanosilver on many organs its effect on the testicles in vivo was not studied. That’s why, the aim of the study was to assess in vivo the effect of nanosilver and silver sulfate on the testicle and to determine the correlation between cytogenetic and cytotoxic parameters. The comparative morphological in vivo evaluation of 2-weeks oral exposure of 4 concentrations (0.1; 5; 50 and 500 mg/l) of silver nanoparticles with size of 14.0 nm, stable arabian gum 1:7 by weight, and of 4 similar concentration of silver sulfate on the testicle of mice. The effect of silver nanoparticles and silver sulfate at the concentration of 500 mg/l is shown to lead to a significant increasing of destructured tubules with undifferentiated and depleted spermatic epithelium, significant decreasing in the number of Leydig cells and decreasing trend in the number of spermatidas, spermatozoons and Sertoli cells, which indicates to the inhibition of spermatogenesis in equal measure for both silver nanoparticles and silver sulfate. The pronounced correlation between the increase in the number of spermatidas with apoptosis and decreasing in the number of tubules with spermatozoon, indicating to the mechanism of gonadotoxic action.

Introduction. A review of the literature on nanotechnology shows the advanced development of hygienic research in the field of nanotoxicology to be of fundamental importance, as it allows creating a basis for the introduction of safe production at the design stage of technological processes. The effects of carbon nanotubes (CNT) on the body are evaluated for the use in many different technical applications, including the alleged possibility of their use as a sorbing material in the water treatment process. Material and Methods. Two-weeks intragastric effect of single-walled carbon nanotubes (oUNT) at doses of 0.05 and 0.5 mg/kg and a complex of vitamins A and E (AE) at a dose of 25.3 mg/kg (vitamin E - 25.2 mg/kg and vitamin A - 0.1 mg/kg) on the liver of male Wistar rats, as well as in the same batch of animals three months after a two-week experiment and a single exposure to oUNT at a dose of 5mg/kg were studied in fifteen groups of animals. oUNT was dissolved in sunflower oil at the rate of 0.5 ml of oil per 100 g of body weight. Results. Two-weeks study of oUNT at doses of 0.05 and 0.5 mg/kg and three months after a single exposure at a dose of 5mg/kg revealed no damage to the nucleus of hepatocytes, but three months after a two-week experiment, when exposed to both doses of oUNT, showed a significant increase in this index, as well as indices of fatty degeneration, only in relation to groups of the two-week exposure. Thus, with two-weeks exposure of oUNT, three months after it, as well as three months after a single exposure at a dose of 5 mg/kg the fatty degeneration develops. The effect of the complex of vitamins AE in the two-weeks experiment and three months after it significantly worsens all the studied parameters in the rat liver, and with its complex effect with oUNT indices remain at the same changed level. Discussion. Probably UNT, getting into the digestive tract, cause pathological changes both in the place of direct contact - in the small intestine and in the remote body - the liver, which is consistent with both our data and previous studies. Conclusion. oUNT in different studied periods of exposure causes the development of fatty degeneration in the liver, and the use of vitamins A and E does not reduce the response of the liver damage.

Introduction. Currently, there is a number of works aimed at studying the effect of carbon nanotubes (UNT), including single-walled (oUNT), on the body and there are studies to find means to reduce their toxic effects, such as compounds with antioxidant properties. Material and methods. In fifteen groups of animals there were studied intragastric effects of two weeks with the count at doses of 0.05 and 0.5 mg/kg and a single dose of 5mg/kg, as well as complex with vitamins A and E (AE) at a dose of 25.3 mg / kg on the small intestine of male Wistar rats, and in the same batch of animals three months later. Results. In a two-week experiment under the influence of oUNT in doses of 0.05 and 0.5 mg/kg there was shown the dose dependence on indices of reducing the number of normal villi with an increase in the number of destructured ones, whereas three months later among the destructured villi were increased in number with apical necrosis. After a single exposure of oUNT at a dose of 5mg/kg three months later, there also was increased the number of villi with apical necrosis. Two-weeks exposure of AE reduced the number of normal villi, increasing the number of degraded forms, and three months later the number of villi with apical necrosis increased. Two-weeks complex exposure of AE and oUN at doses of 0.05 and 0.5 mg/kg reduced the number of normal villi with an increasing number of destructured ones; three months later, as well as with other effects, the pathology developed, increasing the number of villi with apical necrosis. Conclusion. The toxic effect of oUNT and AE on the small intestine of rats with the separate and complex applications, increasing in the recovery period, was proved.