This multicenter study evaluated the effect of harmonizing spectrophotometric (d/8) color measurements by using actual dental materials of clinically relevant thickness and translucency as "calibration tiles." Ten tooth-colored, 1-mm thick, resin-and ceramic-based materials were tested at the coordinating center (CC) and six research sites (RS). Correction factors were calculated for each material and each site to obtain the corrected reflection spectra from the non-corrected data set. Non-corrected (NC) and corrected (C) reflection values were converted into CIEDE2000 and CIELAB color parameters for the following comparisons: CC vs RS NC and CC vs RS C , between RS NC vs RS NC and RS C vs RS C pairs, and among RS separately (α = 0.05). Mean color differences for CC vs RS NC and corresponding CC vs RS C values were ΔE 00 = 4.2 (2.6) and ΔE 00 = 0.7 (0.6), respectively. Corresponding values for RS NC vs RS NC and RS C vs RS C specimen pairs were ΔE 00 = 5.9 (2.9) and ΔE 00 = 1.0 (0.5), respectively. The CIEDE2000 NC vs C comparison for each research site separately ranged from 0.5 to 8.5. Color differences of CC vs RS C and RS C vs RS C pairs decreased 83% to 84.5% as compared with respective color differences for non-corrected research site measurements and was RS dependent (P < .001). The obtained results validated the proposed method of harmonizing color measurements for dental application as a reliable quality control tool for dental research laboratories and industry.