Introduction. Due to the high prevalence of respiratory diseases in children attending pre-school educational settings, the problem of their prevention is especially important. Taking into account the fact that children spend between 6 and 8 hours a day in pre-school educational institutions, one of the risk factors of respiratory diseases is the microbial contamination of indoor air. The analysis of Russian and international scholarly literature revealed the positive impact of phytoncide properties of plants on the quality of indoor air and on the psycho-emotional state of a person, however, due to the lack of experimental evidence, the practices of using healing properties of plants in children's organized groups have not been widely adopted. The results of this study confirm that the rational placement of a certain range of plants in preschool classrooms can become a promising and low-cost direction in the structure of a health-saving approach in the modern system of preschool education. The purpose of the study is to evaluate the effectiveness of phytoncides of a particular range of plants and their placement in reducing microbial contamination of the air in pre-school education settings. Materials and Methods. To study the effect of phytoncide activity of plants the authors monitored the microbial contamination of air in preschool classrooms from two kindergartens in Novosibirsk, with the main focus on the leaf area of the established range of plants and conditions of their placement. For quantitative and qualitative analysis of air microflora composition we used standard differential-diagnostic nutrient media, methods of seeding and calculation of the proportion of total microbial count and facultative microflora. Air sampling points were located in the child's breathing zone (0.5, 1.5 and 3 m away from the plants at 0.8 m height). We monitored the effectiveness of phytoncide properties of plants on the risks of children's disease during the epidemiological rise based on attendance logs. The following theoretical research methods were used: formalization, generalization, comparison and system analysis. Results. It was found that phytoncides reduce microbiological insemination of preschool classrooms where a certain range of phytoncide plants were located. It was determined that the intensity of the phytoncide effect depends on the leaf surface area and their rational distribution, taking into account the effective radius of exposure. The study found a decrease in respiratory diseases among children at preschool educational settings where phytoncide plants were placed in the classrooms. Conclusions. The results of the study can be employed in recommendations for the use of a certain range of plants with pronounced phytoncide activity as one of the components of health-saving conditions in the modern education system.

Introduction. Currently, the problem of keeping schoolchildren healthy is acute. One of the leading risk factors for the health of schoolchildren is nutrition, in the organization of which much attention has recently been paid to the inclusion of special foods that help prevent diet-related diseases. The White Sea brown alga, Laminaria digitata, has been shown to be a natural source of bioactive compounds. However, the mechanisms of the kinetics of health-promoting effects during long-term dietary intake of Laminaria digitata are poorly studied which makes it difficult to solve practical problems in the use of products and dishes containing Laminaria digitata for health purposes. The purpose of this research is to study the biological effects of Laminaria digitata in a 28-day experiment in white Wistar rats to address practical issues of justifying food formulation and food processing for school nutrition, and to assess the risk of side effects. Materials and Methods. The study used dry concentrates of Laminaria digitata. The sample comprised white Wistar rats divided into 4 groups of 10 animals each. The animals were examined on the 14th and 28th days of the experiment using standard methods. The experiment was conducted in accordance with the rules adopted in the European Convention for the Protection of Animals Used for Experimental Scientific Purposes (Strasburg, 1986), after approval by the Ethics Committee of Novosibirsk Research Hygiene Institute. The statistical processing of the research materials was performed using Statistica 10.0. Results. The analysis of the dynamics of the indicators showed the stages of the interaction process of the organism with bioactive substances contained in the brown alga Laminaria digitata. Strengthening of stress-protective, antihypercholesterolemic effects and metabolic function of the liver at the stage of primary reactions is replaced by a significant weakening at the stage of physiological adaptation. The bioavailability of a form of iodine accumulated by the brown algae Laminaria digitata has been experimentally confirmed. The assessment of biological significance consisted of classifying the effects manifested using benefit and safety criteria. Conclusions. Based on the results of the experiment, new knowledge was gained on the criteria for the usefulness and safety of Laminaria digitata algae, which can be used to solve practical problems of proving the quantitative values of the inclusion of Laminaria digitata as an ingredient in recipes and to solve practical problems with Laminaria digitata enriched food production technologies in the development of school meals, the inclusion of which in students' diets minimizes the risk of diet-related diseases.

Introduction. Pentasodium salt of diethylenetriaminepentaacetic acid, which is widely used in the chemical industry as an initiator of polymerization processes, has a wide range of toxic properties. However, the normative hygienic standard for the working area in the whole wide world has not yet been established. Purpose of the study. Experimental substantiation of a large number of one-time MPC of pentasodium salt of diethylenetriaminepentaacetic acid in the environment. Materials and methods. Pentasodium salt of diethylenetriaminepentaacetic acid, CAS No. 140-01-2, outbred male rats weighing 200-240 g. Research in the field of the protection of animals used for scientific purposes (ETS N 123) is aimed at studying the protection of animals used for scientific purposes. Examinations of experimental studies on animals using generally accepted and unified methods. Statistical processing of research materials was carried out using standard application programs Statistica 10.0. Results. The mean lethal dose of diethylenetriaminepentaacetic acid pentasodium salt (DL50) for male white rats is 1702.8±228mg/kg, the acute inhalation action threshold (Limac) is the concentration of 4.62±0.4mg/m3, the irritant action threshold (Limir) - concentration 2.5±0.2 mg/m3, irritating zone (Zir) equals 2.9. Conclusion. A high one-time maximum allowable concentration of the pentasodium salt of diethylenetriaminepentaacetic acid in the environment, equal to 0.7 mg/m3, has been scientifically substantiated and experimentally found. Hazard class 2, limiting indicator of harmfulness - irritant effect. Restrictions. The authors transfer the editors of the exclusive right to natural disasters (publications), other use of the materials of the articles without citing the authors for a specific publication is strictly prohibited. Ethics. The material of the article was approved by the ethics committee at the Federal Budgetary Institution "Novosibirsk Research Institute of Hygiene" of Rospotrebnadzor (No. 2 of January 14, 2022).

For the production of polyacrylamides, pentasodium salt of diethylenetriaminepentaacetic acid is used as a polymerization initiator. At this stage, conditions are created for the contamination of the air substance of the working area with a liquid aerosol. The pentasodium salt of diethylenetriaminepentaacetic acid has a chelating ability to bind to trace elements, in particular zinc, which plays an important role in the proliferation of human cells. Zinc homeostasis is required for many aspects of the immune system and is required for an infectious response. According to the literature, the hygienic standards for the content of pentasodium salt of diethylenetriaminepentaacetic acid in the air of the working area have not yet been established. Objective of the study: to evaluate the irritant effect on lung tissue under conditions of liquid aerosol contamination of pentasodium salt of diethylenetriaminepentaacetic acid. The results of inhalation inoculations confirm that as the level of exposure to pentasodium salt of diethylenetriaminepentaacetic acid increases, the risk of damage to the mucous membranes of the respiratory tract increases. The chelating ability of a substance and its accumulation in the human body can lead to a decrease in the bioavailability of trace elements, in particular, to zinc.

Introduction. The paper presents the results of experimental studies focused on assessing the anti-inflammatory, hypoglycemic and hypolipidemic effects of Stevia rebaudiana Bertoni leaf extract. The study was conducted on an experimental model of "zinc" diabetes in white male Wistar rats and under conditions of acute hyperlipidemia caused by the Tween-80 detergent. The aim of the study was to evaluate the feasibility of using Stevia rebaudiana Bertoni leaf extracts in the diet as a natural sweetener for the organizing nutrition of overweight and diabetic people, including educational settings for children, on the model of induced diabetes mellitus and acute hyperlipidemia in laboratory animals. Materials and Methods. In order to solve the research problem, the authors utilized the following techniques: assessing the biochemical parameters of blood serum, determining the level of glucose in the blood, lipid formula and delayed-type hypersensitivity reaction. Theoretical methods involved reviewing and analyzing Russian and international academic articles. Results. The findings of this study suggest that it is possible to use Stevia rebaudiana Bertoni leaf extracts as a natural sugar substitute in the diet of people with overweight and diabetes mellitus, as well as for prophylaxis in healthy people, including meals for children in educational settings. Conclusions. The article concludes that an immunomodulatory effect and the absence of side effects of the Stevia rebaudiana Bertoni leaf extract confirm its usage in the daily diet of the population.