Background Dengue, the most prevalent arboviral infection disease worldwide affects more than 2.5 billion people. Puerto Rico has endemic transmission of dengue virus (DENV). Chronic diseases like diabetes mellitus (DM) tend to increase susceptibility to infectious diseases. Diabetes mellitus (DM) is also one of the chronic diseases with higher prevalence in the United States (9.1%) and Puerto Rico (12.8%), and its effect on arboviral infections is understudied. Methods A Sentinel Enhanced Dengue Surveillance System was established in a tertiary hospital in the southern region of Puerto Rico. Study aims are to describe and compare the clinical course and prognosis of dengue in patients with and without the DM co-morbidity. A retrospective case-control study (2012-2015) was performed, where 1,005 participants with confirmed dengue were identified. Results In those with dengue, we examined 57 cases with DM and 171 controls without DM on presentation. Mean age of participants was 40 years old, 31 (54.4%) were males. Relevant clinical features of cases were high blood pressure (HBP) in 51%, muscle pain (70.2%) and joint pain (63.2%). Laboratory results were thrombocytopenia (40%), high creatinine >1.2mg/dl (18.5%), high BUN >20mg/dl (16.6%) and low albumin < 3.4 g/dl (28.7%). For controls, clinical and laboratory results were HBP in 26.9%, muscle pain (64.3%) and joint pain (56.7%); thrombocytopenia (38.6%), high creatinine (4.7%), high BUN (1.8%) and low albumin (21.6%). ANOVA test compared the means of clinical features and laboratory parameters between the case and control groups. The results indicate that high blood pressure, high creatinine and high BUN were significantly more common in cases than in controls (P < 0.01). Cases that had high creatinine (OR = 2.03 [CI: 0.01 – 6.08]), high BUN (OR = 1.30 [CI: 0.93 – 1.81]) and high blood pressure (OR = 1.02 [CI: 0.93 – 1.11]) were more likely to present bleeding, a warning sign for severe dengue. Also, diabetic cases that presented thrombocytopenia were more likely to be admitted to the hospital (OR = 2.64 [CI: 0.36 – 19.37]). Conclusion Understanding the clinical manifestation of dengue in patients with DM can increase clinician awareness of the importance of this co-morbidity in order to implement preventive measures and improve clinical outcomes. Disclosures All Authors: No reported disclosures

Background Expanding and providing access to early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) through testing community-based strategies among socially vulnerable communities (SVC) are critical to reducing health disparities. The Epidemiological Intelligence Community Network (EpI-Net) community-based intervention sought to increase coronavirus 2019 (COVID-19) testing uptake and prevention practices among SVC in Puerto Rico (PR). We evaluated EpI-Net’s community leaders’ capacity-building component by assessing pre-post COVID-19 public health workshops’ tests’ score changes and satisfaction among trained community leaders. Methods A total of 24 community leaders from SVC in PR have completed four community workshops. Pre- and post-assessments were completed as part of the health promotors training program to evaluate participants’ tests score changes and satisfaction outcomes. Results Preliminary results showed: (1) high intervention retention levels of community leaders (85.7% acceptance rate); (2) change in post-test scores for community engagement strategies (p = 0.012); (3) change in post-test educational scores in COVID-19 prevention practices (p = 0.014); and (4) a change in scores in public health emergency management strategies (p < 0.001). Conclusions The overall workshop satisfaction was 99.6%. Community leaders have shown the importance of community capacity building as a key component for intervention feasibility and impact. Trial registration Our study was retrospectively registered under the ClinicalTrial.gov ID NCT04910542.