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[1] A long-duration pumping test performed in the conduit of a mixed flow karst system (MFKS) is analyzed and interpreted. It constitutes a unique experiment of catchment wide response of a karst system, with drawdowns measured both in the pumped conduit and in the matrix. A modeling approach is proposed for this interpretation. The developed double continuum model consists of two reservoirs -karst conduits and the surrounding carbonate rocks -between which flow exchange is modeled using the superposition principle and the hypothesis of Darcian flow in the matrix considered as an equivalent porous media. The karst conduits are assumed to have an infinite hydraulic conductivity. Model calibration results in a very good match (relative root mean square [rRMS] = 2.3 %) with drawdown measured at the pumping well (karst conduit). It shows that the matrix hydrodynamic parameters (hydraulic conductivity and storativity) have a greater influence on the drawdown than the storage capacity of the conduit network. The accuracy of the model relies mostly on a very good knowledge of both pumping rate and natural discharge at the spring (with and without pumping). This type of approach represents an advance in double continuum modeling of karst systems. It also provides a methodology for the management of water resources from karst aquifers.

Abstract. Bacteriophages are increasingly used as tracers for quantitative analysis in both hydrology and hydrogeology. The biological particles are neither toxic nor pathogenic for other living organisms as they penetrate only a specific bacterial host. They have many advantages over classical fluorescent tracers and offer the additional possibility of multi-point injection for tracer tests. Several years of research make them suitable for quantitative transport analysis and flow boundary delineation in both surface and ground waters, including karst, fractured and porous media aquifers. This article presents the effective application of bacteriophages based on their use in differing Swiss hydrological environments and compares their behaviour to conventional coloured dye or salt-type tracers. In surface water and karst aquifers, bacteriophages travel at about the same speed as the typically referenced fluorescent tracers (uranine, sulphurhodamine G extra). In aquifers of interstitial porosity, however, they appear to migrate more rapidly than fluorescent tracers, albeit with a significant reduction in their numbers within the porous media. This faster travel time implies that a modified rationale is needed for defining some ground water protection area boundaries. Further developments of other bacteriophages and their documentation as tracer methods should result in an accurate and efficient tracer tool that will be a proven alternative to conventional fluorescent dyes.

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