Rutting is one of the most common distresses in asphalt pavements in Zambia. The problem is particularly prevalent at intersections, bus stops, railway crossings, police checkpoints, climbing lanes and other heavily loaded sections, where there is deceleration, slow moving or static loading. The most widely used methods to identify the source of rutting among flexible pavement layers are destructive methods; field trenching and coring methods. The Transverse Profile Analysis method (TPAM), which is a non-destructive method, was suggested by White et al. in 2002 as an alternative method, to avoid the expensive and destructive nature of the traditional methods. In this method, data from the transverse profile of the rutted section is used to deduce the layer of the pavement structure responsible for rutting failure. This study used the TPAM to determine the layers of pavement responsible for rutting on sections of the Chibuluma and Kitwe-Chingola Roads in Zambia. The method was first validated using the trenching method on the Kitwe-Ndola Road. Results from the TPAM showed good comparability with those from the trenching method. It was established that most of the rutting emanated from the surfacing layer. This is consistent with recent research indicating that most rutting occurs in the upper part of the asphalt surfacing. It was also established that the TPAM was a simpler, faster and less costly method of determining the source of rutting failure compared to the traditional methods.