Biosensors are analytical devices that sense the presence of a biological component in the vicinity and generate signals that can be easily detected using a physicochemical detector. With the advent of biosensors, conventional methodologies such as the detection of contaminants in pharmaceutical industries and the diagnosis of diseases via biomarkers have been accelerated to a great extent. The review highlights the use of Amperometric biosensors in three important industries namely, the food, fermentation, and dairy industries, and highlights the potential use of these biosensors in various domains of these industries. These industries can employ the use of amperometric biosensors to control and monitor the presence of various metabolites and constituents that may either be of economic importance or toxic to the consumers. The continuous monitoring of these metabolites is of prime importance as they ultimately determine the quality of the end product. The review also gives a comprehensive description of the modifications done in the amperometric biosensors, using specific biomolecules and chemicals, along with their associated analytical features.