Objective The objective of the study was to evaluate the computer workstation settings and the prevalence of musculoskeletal (MSK) related problems in computer users of bank. Methods A cross-sectional study was conducted among100 computer users of State Bank of Pakistan, Faisalabad and National Bank of Pakistan, Faisalabad from March to December 2017. Inclusion criteria of the study were male and female bank workers, age between 25–60 years who use desktop computers for at least 3 h a day and had at least 6 months working experience. Cornell Musculoskeletal Discomfort Questionnaire (CMDQ) was used to determine prevalence of MSK problems while Rapid Office Strain Assessment (ROSA) tool was used to evaluate Computer Work-Station (CWS) ergonomic parameters. The data was analyzed by descriptive statistics and variant correlation using SPSS version 20. Results The mean CMDQ was higher (43.0 ±33.6) in female than male (16.9±20.8) and there was significant association between gender and total discomfort (P=0.001). The discomfort was higher among females then males. The body parts reported to have the most significant levels of discomfort was the neck and head (mean 3.595±7.49), lower back (2.75±5.39) and right shoulder (mean 2.15±4.32).About 40% subjects suffered from neck pain while the remaining body parts having discomfort were right shoulder (39%), left shoulder (37%), and low back (36%).Almost 90% of the participants had ROSA score greater than 5 that shows that most individuals were at higher risk of ergonomics hazardous. Conclusion The results of the present study demonstrated that MSK discomfort is common among computer users of bank employees and neck, shoulder and lower back region were at greater risk. MSK discomfort was higher in females. Workers are exposed to ergonomic hazards and need immediate improvement.