The purpose of this study was to investigate the level of scientific and civic cultural literacy of the American University of Madaba (AUM) students and its relationship to personal variables. The sample of the study consisted of 437 students registered for the first semester of the academic year 2019-2020 at AUM and  sat for a scientific and civic test, the main research tool. The analysis of data collected showed that AUM students are culturally literate in the science field where the scores are at a high level. However, it was a little less in civics as it scored medium up to a high level. Further,  there were statistically significant differences at (α = 0.05) in the scientific field in favor of the females, the international curriculum and health sciences faculty but not in the civics field. It is recommended that further research studies should be conducted on other fields of cultural literacy.