Forty soil samples collected from different sites of Hillah city /Iraq.Sixty seven Streptomyces isolates were obtained ,purified and identified according to morphological properties and biochemical tests. All of these isolates were screened fortheir antimicrobial activity,in addition to production of some important enzymes such as amylase, protease ,cellulase and L-asparaginase.The results exhibited that five isolates ;Streptomyces sp.(SA.2 ,SK.5 , SM.42 , SS.50 and SI.63) have such potential. The most active isolate in production of thestudied enzymes was SM.48.On the other hand, it has less antibacterial activity againsttestmicroorganismsEscherichia coli ,Pseudomonas aeruginosa,Staphylococcusaureus ,Moganella sp. and Klebseillapnumoniae.While the isolate Streptomyces sp.SS.50 has no activity in enzyme production ,but it has a good antibacterial activity due to inhibition zone diameter (mm) ;19 ,18 ,16 ,14 and 16 mm towards the previous mentioned bacterial species , respectively , in addition to that ,it also gave clear zone at 16 and 12 mmagainst Candida albicans and Candida glabrata , respectively.

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