Background: The use of supplements, herbal extracts, and exercise training for the treatment of diseases and metabolic disorders has increased among people. Thus, the aim of this study was the study of The effect of a period of Pilates and the supplementation of Royal Jelly on inflammatory indexes of diabetic obese women. Materials and Methods:In this quasi-experimental study 44 volunteer diabetic obese women participated and They were randomly divided into Four groups of 11 controls, Royal Jelly, Pilates, Pilates + Royal Jelly . Pilates exercise training was performed during 8 weeks (3 sessions per week, 60 min per session). The subjects consumed a supplement group of 1000 mg of royal Jelly daily for 8 weeks. covariance analysis test were used to examine the in-group differences and between the research groups. Results:The results showed that eight weeks of Pilates and Royal Jelly Supplements significantly reduced the TNF-α, CRP and IL-6 diabetic women(P=0.001). In addition, serum glucose and serum insulin also decreased significantly in during eight weeks of Pilates and supplementation of the royal Jelly(P=0.001). Vo2max significantly Increase in training, training + supplement groups (P=0.001). Conclusion:According to obtained results, it appears that Pilates training plus Royal Jelly extract consumption have better effect on serum inflammatory factors in obsess women with diabetes type 2