A noninvasive investigation to ascertain the platelet (PLT) count was conducted on 44 hospitalized dengue hemorrhagic fever (DHF) subjects, male and female aged between 3 and 14 years using bioelectrical phase angle (BPhA). Among the 44 subjects, 30 subjects were confirmed to be non-structural protein-1 (NS1) positive at the time of admission, whose blood investigations such as hematocrit (HCT) level, PLT count, aspartate aminotransferase (AST) level and alanine aminotransferase (ALT) level were performed for the classification of risk as low-risk (LR) and high-risk (HR) DHF. It was found that the BPhA of the body reflects a linear correlation with the PLT count. To provide a better and more accurate estimate of PLT, a dual-frequency method is proposed to calculate the phase angle of the total body. The resistance at 5 kHz and reactance at 100 kHz were used to estimate the phase angle of the total body. The statistical analysis identified that the PLT count estimated using the proposed dual-frequency method shows a good correlation with the blood investigation results. In addition, statistical analysis of the proposed method on other fever subjects indicated a significant difference with DHF.

A noninvasive investigation to ascertain the hematocrit (HCT) or packed cell volume (PCV) was conducted on 44 hospitalized dengue hemorrhagic fever (DHF) subjects, male and female aged between 3 and 14 years using bioelectrical impedance analysis (BIA). Among the 44 subjects, 30 subjects were confirmed to be non-structural protein-1 (NS1) positive at the time of admission, whose blood investigations such as HCT level, platelet (PLT) count, aspartate aminotransferase (AST) level and alanine aminotransferase (ALT) level were taken for the classification of risk as low risk (LR) and high risk (HR) DHF. Electrical conductivity of blood reflects a linear correlation with HCT. To provide a better and more accurate estimate of HCT, a dual frequency method is proposed to calculate the conductivities of plasma and blood cells. The resistance at 100 kHz is used to estimate the conductivity of blood cells and the impedance at 5 kHz to estimate the conductivity of plasma. Statistical analysis reveals that the HCT estimated using the proposed dual frequency method shows a significant difference with a single frequency (50 kHz) estimate of HCT and also shows a good correlation with the blood investigation results. In addition, statistical analysis of the proposed method on different fever subjects indicates a significant difference with DHF.

A noninvasive investigation to ascertain the severity of dengue was conducted on 44 hospitalized dengue hemorrhagic fever (DHF) subjects, male and female aged between 3 and 14 years using bioelectrical impedance analysis. Among the 44 subjects, 30 subjects were confirmed with NS1 positive at the time of admission, whose blood investigations such as haematocrit (HCT), platelet (PLT) count, aspartate aminotransferase (AST) level and alanine aminotransferase (ALT) level was taken for classification of risk under low risk and high risk DHF. For comparison, BIA of 53 healthy controls was also taken. To provide a better and accurate estimate, a dual frequency method is proposed to calculate body fluid volumes such as total body water (TBW), extracellular fluid (ECF) and intracellular fluid (ICF). The impedance at 100 kHz is used to estimate TBW while impedance at 5 kHz to estimate ECF. Statistical analysis identifies that the ratio of ECF/ICF estimated using the proposed dual frequency method, shows significant difference between control and DHF risk groups and also shows good correlation with the blood investigation results. In addition, statistical analysis of proposed ECF/ICF method on other fever subjects indicate significant difference with DHF.

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