Nuclear segmentation in digital microscopic tissue images can enable extraction of high-quality features for nuclear morphometrics and other analysis in computational pathology. Conventional image processing techniques, such as Otsu thresholding and watershed segmentation, do not work effectively on challenging cases, such as chromatin-sparse and crowded nuclei. In contrast, machine learning-based segmentation can generalize across various nuclear appearances. However, training machine learning algorithms requires data sets of images, in which a vast number of nuclei have been annotated. Publicly accessible and annotated data sets, along with widely agreed upon metrics to compare techniques, have catalyzed tremendous innovation and progress on other image classification problems, particularly in object recognition. Inspired by their success, we introduce a large publicly accessible data set of hematoxylin and eosin (H&E)-stained tissue images with more than 21000 painstakingly annotated nuclear boundaries, whose quality was validated by a medical doctor. Because our data set is taken from multiple hospitals and includes a diversity of nuclear appearances from several patients, disease states, and organs, techniques trained on it are likely to generalize well and work right out-of-the-box on other H&E-stained images. We also propose a new metric to evaluate nuclear segmentation results that penalizes object- and pixel-level errors in a unified manner, unlike previous metrics that penalize only one type of error. We also propose a segmentation technique based on deep learning that lays a special emphasis on identifying the nuclear boundaries, including those between the touching or overlapping nuclei, and works well on a diverse set of test images.

Generalized nucleus segmentation techniques can contribute greatly to reducing the time to develop and validate visual biomarkers for new digital pathology datasets. We summarize the results of MoNuSeg 2018 Challenge whose objective was to develop generalizable nuclei segmentation techniques in digital pathology. The challenge was an official satellite event of the MICCAI 2018 conference in which 32 teams with more than 80 participants from geographically diverse institutes participated. Contestants were given a training set with 30 images from seven organs with annotations of 21,623 individual nuclei. A test dataset with 14 images taken from seven organs, including two organs that did not appear in the training set was released without annotations. Entries were evaluated based on average aggregated Jaccard index (AJI) on the test set to prioritize accurate instance segmentation as opposed to mere semantic segmentation. More than half the teams that completed the challenge outperformed a previous baseline [1]. Among the trends observed that contributed to increased accuracy were the use of color normalization as well as heavy data augmentation. Additionally, fully convolutional networks inspired by variants of U-Net [2], FCN [3], and Mask- RCNN [4] were popularly used, typically based on ResNet [5] or VGG [6] base architectures. Watershed segmentation on predicted semantic segmentation maps was a popular post-processing strategy. Several of the top techniques compared favorably to an individual human annotator and can be used with confidence for nuclear morphometrics.

Emotion recognition technology through analyzing the EEG signal is currently an essential concept in Artificial Intelligence and holds great potential in emotional health care, human-computer interaction, multimedia content recommendation, etc. Though there have been several works devoted to reviewing EEG-based emotion recognition, the content of these reviews needs to be updated. In addition, those works are either fragmented in content or only focus on specific techniques adopted in this area but neglect the holistic perspective of the entire technical routes. Hence, in this paper, we review from the perspective of researchers who try to take the first step on this topic. We review the recent representative works in the EEG-based emotion recognition research and provide a tutorial to guide the researchers to start from the beginning. The scientific basis of EEG-based emotion recognition in the psychological and physiological levels is introduced. Further, we categorize these reviewed works into different technical routes and illustrate the theoretical basis and the research motivation, which will help the readers better understand why those techniques are studied and employed. At last, existing challenges and future investigations are also discussed in this paper, which guides the researchers to decide potential future research directions.

Detecting various types of cells in and aroundthe tumor matrix holds a special significance in characterizing the tumor micro-environment for cancer prognostication and research. Automating the tasks of detecting, segmenting, and classifying nuclei can free up the pathologists' time for higher value tasks and reduce errors due to fatigue and subjectivity. To encourage the computer vision research community to develop and test algorithms for these tasks, we prepared a large and diverse dataset of nucleus boundary annotations and class labels. The dataset has over 46,000 nuclei from 37 hospitals, 71 patients, four organs, and four nucleus types. We also organized a challenge around this dataset as a satellite event at the International Symposium on Biomedical Imaging (ISBI) in April 2020. The challenge saw a wide participation from across the world, and the top methods were able to match inter-human concordance for the challenge metric. In this paper, we summarize the dataset and the key findings of the challenge, including the commonalities and differences between the methods developed by various participants. We have released the MoNuSAC2020 dataset to the public.

Existing deep learning technologies generally learn the features of chest X-ray data generated by Generative Adversarial Networks (GAN) to diagnose COVID-19 pneumonia. However, the above methods have a critical challenge: data privacy. GAN will leak the semantic information of the training data which can be used to reconstruct the training samples by attackers, thereby this method will leak the privacy of the patient. Furthermore, for this reason, that is the limitation of the training data sample, different hospitals jointly train the model through data sharing, which will also cause privacy leakage. To solve this problem, we adopt the Federated Learning (FL) framework, a new technique being used to protect data privacy. Under the FL framework and Differentially Private thinking, we propose a Federated Differentially Private Generative Adversarial Network (FedDPGAN) to detect COVID-19 pneumonia for sustainable smart cities. Specifically, we use DP-GAN to privately generate diverse patient data in which differential privacy technology is introduced to make sure the privacy protection of the semantic information of the training dataset. Furthermore, we leverage FL to allow hospitals to collaboratively train COVID-19 models without sharing the original data. Under Independent and Identically Distributed (IID) and non-IID settings, the evaluation of the proposed model is on three types of chest X-ray (CXR)images dataset (COVID-19, normal, and normal pneumonia). A large number of truthful reports make the verification of our model can effectively diagnose COVID-19 without compromising privacy.