Benzoylpyridine and nitro benzoic acid hydrazides were used to examine novel Schiff base Co(II), Mn(II), Fe(II), and Cu(II) complexes. Elemental analysis, molar conductance, magnetic measurements and other methods that like FT-IR, UV–Vis and 1H NMR studies have been used to deduce the nature of bonding and structure of these complexes. These studies revealed that the Co(II), Mn(II), Fe(II) and Cu(II) coordinated by four donor atoms in all the complexes. Free radical scavenging activity was determined according to the elimination of radicals from 2,2-diphenyl-1-picrylhydrazyl (DPPH). These compounds were compared to known antibiotics ciprofloxacin and fluconazole in their ability to inhibit the growth of pathogenic bacteria Bacillus subtilis (as gramme positive bacteria), Pseudomonas sp. (as gramme negative bacteria), and fungus Rhizoctonia sp., Alternaria sp., and Fusarium oxysporum. As an antibacterial and antioxidant agent, the complexes are more effective than the ligand. The copper (II) complex has been discovered to be the most effective antibacterial and antioxidant agent.

Aim: The main objective of the study is to estimate the effect of extraction techniques and antimicrobial activity of different solvent extract of A. mexicana leaves and roots. Study Design: The research is experimental in nature. Place and Duration of Study: Department of Chemistry and Microbiology at CCSHAU, Hisar, between late 2020 and January 2022. Methodology: The leaves and roots of A. mexicana were harvested for this study. Shade dried roots and leaves were cut into small pieces of 2-3 inches and processed into powder using a mixer grinder. Soxhlet extraction and microwave-assisted extraction procedures were used to extract leaves and roots in solvents such as acetone, methanol, and water. The antimicrobial activity of the roots and leaves  extracts were evaluated against Gram +ve bacteria (Xanthomonas campesteris, Bacillus cereus, Staphylococcus aureus) and fungal species (Fusarium oxysporum, Macrophomina phaseolina and Candida albicans) and their zones of inhibition in mm are measured by Agar well diffusion method. Results: Soxhlet extraction technique gave better extract yield 12.19 g /100 g and 8.54 g / 100 g while microwave-assisted extraction gave 8.88 g / 100 g and 6.94 g / 100 g for leaves and roots respectively. The methanoilc root and leaves extracts exhibited higher antimicrobial activity followed by acetone and water extracts. Conclusion: The result of the investigation showed that extraction techniques considerably affected extraction yield and antimicrobial activity. Soxhlet extraction is better one extraction method among these two and methanolic extract of leaves was found to be good antimicrobial followed by acetone and aqueous.