Thirty Damascus does aged 1.5-2 years and weighed 46.9±1.64 kg were used to define the influence of Origanum Vulgare oil or N-acetyl cysteine supplementation on reproductive performance, milk yield and composition, growth performance in addition to changes in some blood metabolites during late pregnancy and suckling periods of Damascus does. Does were randomly divided into three equal groups (10 each) and،fed basal ration according to NRC (1981). The first group, acted as control (G1), received a basal diet made up of 60% concentrate feed mixture (CFM), 20% berseem hay, and 20% rice straw. While the second (G2) and third (G3) groups consumed the same basic ration and supplemented daily with Origanum vulgare oil (OV oil) at a level of 1m. / kg concentrate (G2) and 0.3 gm / kg live body weight of N-acetyl-cysteine (NAC) after being dissolved in 200 ml of water and administered once daily for seven consecutive days during pregnancy and the same period after birth (Start before the first day of breastfeeding) (G3).Results indicated that both treated groups, during late pregnancy and suckling periods, showed improve in fecundity, prolificacy, kids born per does joined, kids born or weaned per does kidded and kg born and weaned per doe kidded, taking in consideration that the flock have history of high mortality rates and still birth which indicated in the values presented for the control group. The high mortality rate of control group (30%) efficiently reduced by NAC (6.67%) while lightly reduced by OV oil (22.22%).Daily milk yield of both treated groups were significantly higher than control group (G1). Fat, protein and lactose percentages for both treated groups also were significantly (P≤0.05) higher compared to the control group.Either Origanum Vulgare oil or N-acetyl cysteine supplement led to a significant increase in weaning weights, daily gain and total gain of kids, while birth weight value does not show any differences between treatments. The best weights occurred with N-acetyl cysteine.Origanum Vulgare oil or N-acetyl cysteine significantly (P<0.05) increased glucose, ALT and Vit C, while, the concentration of urea and AST significantly (P<0.05) decreased as result of Origanum Vulgare oil or N-acetyl cysteine treatment during late pregnancy and lactation periods as compared to the control does.

his work aimed to assess the influence of removal of seminal plasma (RSP) and caffeine supplementation upon quality and fertilizing ability of Egyptian buffalo-bulls' spermatozoa post 6 days of hypothermic preservation at 5ºC. Semen samples were collected from five buffalo-bulls pooled, then separated into two main parts. First part was centrifuged (washed) twice to remove the seminal plasma, then the sperm plugs were re-suspended in camel skim milk extender to a volume equal to that of semen pre-washing. Second part was non-centrifuged (unwashed). Both washed and unwashed semen were diluted (1:4) with camel skim milk extender containing 10% egg yolk supplemented with caffeine at different concentrations (0,5,10 and 15 mM) and gradually cooled to 5°C then preserved at this temperature for 6 days. Data elaborated that RSP enhanced (P<0.01) sperm motility, head-to-head agglutinated spermatozoa and conception rate percentages, while dead, abnormalities and acrosomal damage percentages of spermatozoa , as well as, leakage of ALT, AST, hyaluronidase and ALP enzymes to extracellular fluids of spermatozoa were reduced (P<0.01) post-RSP . The best (P<0.01) semen characteristics, enzymatic activities and fertilizing efficiency of buffalo-bulls' spermatozoa were registered with washed diluted buffalo-bulls semen supplemented with 10 mM caffeine. Conclusively, removal of seminal plasma is a simple, effective, and easily practicable technique, which supplies a helpful alternative to semen preserved in liquid status, especially when administrated with 10mM caffeine for optimizing quality and fertilizing efficiency of buffalo spermatozoa that can be useful for artificial insemination in Egyptian buffaloes.