Buffalo hide is potential as an alternative raw material for making halal collagen to substitute those made from cow and porcine. However, the complexity of buffalo hide is higher than cattle hide and pigskin. Acetic acid is a weak acid that is able to extract collagen properly. The study aimed to determine the effect and the interaction of various concentrations of acetic acid and extraction time on collagen quality, including yield, water content, and collagen's characteristics based on molecular weight and functional groups. The main research material was 3 (three) sides of male buffalo (swamp buffalo) skin aged 3-4 years. This study was an experimental research with factorial completely randomized design, which consisted of the factor of acetic acid concentrations (3%, 5%, 7%) and the factor of extraction times (48 hours and 72 hours). All treatments were replicated four times. The optimum result of buffalo hide collagen extraction was using a concentration of 1 M acetic acid with 72 hours extraction time that produced collagen with the yield of 1.42±0.27%, based on the concentration factor, and the yield of 1.70 ± 0.35%, based on the extraction time factor, 4.19±0.14% water content, and it was identified that collagen has not yet been degraded into gelatin based on molecular weight and functional groups.