The applications of dielectrophoretic (DEP) techniques for the manipulation of cells in a label-free fashion within microfluidic systems continue to grow. However, a limited number of methods exist for making highly sensitive separations that can isolate subtle phenotypic differences within a population of cells. This paper explores efforts to leverage that most compelling aspect of DEP—an actuation force that depends on particle electrical properties—in the background of phenotypic variations in cell size. Several promising approaches, centering around the application of multiple electric fields with spatially mapped magnitude and/or frequencies, are expanding the capability of DEP cell separation.

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