Endocrine disruptor chemicals are exogenous molecules that generate adverse effects on human health by destabilizing the homeostasis of endocrine system and affecting directly human reproductive system by inhibiting or activating oestrogenic or androgenic receptors. Endocrine disruptor chemicals generate transgenerational epigenetic problems, besides being associated with male infertility. Epidemiological data indicate that the increase in reproductive problems in males in the last 50 years is correlated with the increase of endocrine disrupting chemicals in the environment, being associated with a decrease in semen quality and direct effects on spermatozoa, such as alterations in motility, viability and acrosomal reaction, due to the generation of oxidative stress, and have also been postulated as a possible cause of testicular dysgenesis syndrome. Diverse antioxidants, such as C and E vitamins, N-acetylcysteine, selenium and natural vegetable extracts, are among the alternatives under study to counter the effects of endocrine disruptor chemicals. In some cases, the usage of them has given positive results and the opposite in others. In this review, we summarize the recent information about the effects of endocrine disruptor chemicals on male reproduction, on sperm cells, and the results of studies that have tested antioxidants as a strategy to diminish their harmful effects.

Infertility, which has been defined as the inability to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse or due to an impairment of a person's capacity to reproduce either as an individual or with her/ his partner ("Definitions of infertility and recurrent pregnancy loss, 2020"), is a highly prevalent condition (Inhorn & Patrizio, 2015) that affects a fifth of the couples that try to conceive worldwide (Szkodziak et al., 2016; Tadros & Sabanegh, 2017). The percentage of infertility attributable to the male factor ranges between 20% and 70% around the world and at least 30 million men worldwide are infertile (Agarwal et al., 2015). However, in most cases of male infertility, the aetiology is still unknown and classified as idiopathic (Ramalingam et al., 2014). Despite this, among the possible causes that lead to male infertility, reactive oxygen species (ROS) have emerged as one of the main ones due to their toxic and deleterious effects on sperm quality and function

Immunohistochemistry allows in situ detection of cell and extracellular components through specific antibodies. The objective was to compare the immunohistochemical expression patterns of the S-100, HMB-45 and MART-1 proteins for differential diagnosis of malignant melanoma and melanocytic nevus in human skin biopsies. Thirty-nine biopsies of human tissue were used. They were divided into two groups: 19 in malignant melanoma and 20 in melanocytic nevi. Next, the samples were fixed with paraformaldehyde and processed following the protocol for inclusion. Then, immunohistochemical staining was performed. Finally, the histological and qualitative analysis of the samples was carried out. S-100, HMB-45, and MART-1 markers showed positive immunoreaction in melanoma biopsies. HMB-45 marker was generally present with weaker expression than S-100 and MART-1 in melanocytic nevus biopsies. No expression pattern was observed which specifically associates one or more markers with some types of histopathological diagnosis. Immunohistochemistry is fundamental in differential diagnosis of melanomas and melanocytic nevi. However, there is no antibody or set of antibodies which allows unequivocal diagnosis between melanoma and nevus. It is therefore necessary to analyze with care the expression pattern and location of the lesion using standard morphological characteristics.