The purpose of this study was to evaluate and compare the effect of Reciproc Blue (RPCB), XP‐endo Shaper (XPS), and WaveOne Gold (WOG) single‐files on dentinal microcrack formation using micro‐computed tomography (micro‐CT). Twenty‐four mesial roots (24 mesio‐buccal and 24 mesio‐lingual canals) of mandibular molar teeth were prepared using RPCB, XPS, and WOG files (n = 8/each group). The samples were scanned with micro‐CT in the pre‐ and post‐preparation. Then, before preparation and after preparation cross‐sectional images of the teeth were evaluated to detect the presence of microcracks. For each group, the number of microcracks was calculated as a percentage rate. The data were statistically analyzed using McNemar at 5% significance level (p < .05). Dentinal microcracks were observed in 25.99% (2,103 of 7,813), 31.99% (2,482 of 7,758), and 36.66% (2,836 of 7,731) of cross‐sectional images of the XPS, WOG, and RPCB groups, respectively. In all the groups, all the dentinal microcracks seen in the post‐treatment cross‐sectional images were present in the corresponding pre‐treatment images. Within the limitations of this study, the XPS, WOG, and RPCB files did not cause new dentinal microcrack formation or propagation of existing dentinal microcracks.
The study aimed to determine the effect of different irrigation activation systems on the amount of apical debris extrusion in mandibular molar teeth with curved root canals. Sixty extracted mandibular molar teeth were instrumented with HyFlex EDM system and divided into four groups according to irrigation techniques: passive ultrasonic irrigation (PUI), EDDY, photon‐initiated photoacoustic streaming (PIPS) and manual irrigation (MI). After the final irrigation, the extruded irrigants were collected into Eppendorf tubes of known weights. The amount of extruded debris was obtained by subtracting the weight from the initial weight of the tube. The Kruskal–Wallis test was used for statistical analysis. EDDY caused significantly more debris extrusion than the other irrigation activation systems (P < 0.001). PUI caused less apical debris extrusion but there was no statistical difference between PUI, PIPS and MI (P > 0.05). The EDDY activation system was associated with the highest amount of debris extrusion in curved root canals.
The aim of this study was to compare the cyclic fatigue resistance of rotary NiTi files, produced with four different manufacturing methods on specially designed dynamic models that simulated clinical conditions. In this study, 120 files, consisting of 30 files for Typhoon, ProTaper Next, RaCe, and Twisted Files nickel titanium rotary systems were used. The 30 files of each group were divided into three subgroups to be used in artificial canals with a 60° angle of curvature and radii of curvature of 2, 5, and 8 mm (n = 10). All files were rotated in the artificial canals until fracture occurred and the number of cycles to fracture was calculated. The data were analyzed using one‐ and two‐way analyses of variance and Tamhane multiple comparison tests. In all three groups, Typhoon instruments had the highest number of cycles to failure than the RaCe, ProTaper Next, and Twisted Files instruments, and the difference statistically significant (p < .05). There were no significant differences between the RaCe, ProTaper Next, and Twisted Files groups (p > .05). The CM wire Typhoon system was significantly more resistant to cyclic fatigue compared to the other file systems in all three artificial canals. When the fracture resistance of an instrument in three different artificial canals was compared, the mean NCFs decreased as the radius of the curvature of the canal decreased from 8 to 2 mm. Manufacturing method is one of the most important factors on cyclic fatigue resistance, also the radius of curvature effects the cyclic fatigue.
The aim of this study was to evaluate the antibacterial e cacy of copper added chitosan nanoparticles (CU-CNPs) as an irrigation solution with different irrigants in terms of eliminating Enterococcus Faecalis (E. faecalis) from the root canals. Fifty mandibular premolar teeth were prepared and infected with E. faecalis for 21 days. After the incubation period, samples were randomly divided into a control group irrigated with distilled water and 4 experimental groups (n = 10) irrigated with as follows, %6 NaOCl, %6 NaOCl + %9 editronate (HEBP), Chitosan nanoparticles (CNPs), and CU-CNPs. To calculate the proportion of dead E. faecalis cell volume, stained using LIVE/DEAD BacLight Bacterial Viability Kit and were scanned using confocal laser scanning microscope (CLSM). All the irrigation solutions signi cantly (P < .05) killed the bacteria in the canal, except for the control group. CU-CNPs solution killed the highest (P < .05) number of bacteria compared with the other experimental groups. No signi cant difference was found between CNPs, NaOCl + HEBP and NaOCl in terms of antibacterial activity. CU-CNPs solution was exhibited higher antibacterial e cacy against E. faecalis.
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