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ObjectiveTo evaluate the effect of litter size standardization at birth on adult metabolic parameters.MethodsFemale Wistar rats became pregnant at 12 weeks of age. Offspring were divided into control(C), G8(8) and G4(4 newborns/litter) groups. At 12 weeks of age, food intake (FI,g/day), glucose (G,mg/dL), insulin (I,pM), cholesterol (Chol,mg/dL), triacylglycerols (TAG,mg/dL) levels, white and brown adipose tissue (BAT) masses (g/100g) adiposity index (AI,% body weight) were determined.Results(mean±SEM p<0.05, n=6 to 32): Male C G8 G4 AI 4±0.1 4±0.1 5±0.51 FI 26±0.7 24±0.9 17±21 BAT 0.13±0.01 0.11±0.003 0.09±0.011 G 109±3 100±3 120±41 I 129±13 126±10 203±361 Chol 81±32 66±3 69±5 TAG 50±4 67±53 67±43 BP 127±44 101±6 121±124 p<0.05 vs C and G8; p<0.05 vs G8 and G4; p<0.05 vs C; p<0.05 vs G8 Female C G8 G4 AI 5±0.3 5±0.3 7±0.71 FI 17±0.82 15±0.8 16.6±0.92 BAT 0.16±0.022 0.13±0.007 0.11±0.0051 G 92±42 89±2 104±41 I 50±62 71±9 106±161 Chol 57±3 62±4 66±53 TAG 46±5 63±53 65±73 BP 125±74 99±6 106±82 p<0.05 vs C and G8; p<0.05 vs G8; p<0.05 vs C; p<0.05 vs G8 and SG4 ConclusionResults suggest that changes in litter size is associated with energy balance, carbohydrate and lipid disorders, insulin resistance and alterations in blood pressure in adult male and female.Supported by FAPESP

AGRADECIMENTOS Professor Doutor Joel Claudio Heimann,Por me aceitar como sua aluna e me ensinar à importância da pesquisa científica. Pela paciência e dedicação incansável, pelo exemplo de competência e ética profissional, pela orientação impecável. Doutora Luzia Naôko Shinohara Furukawa, pela constante dedicação e amizade, me ensinando e ajudando em todos os experimentos, sem os quais não conseguiria realizar sozinha.Msc. Ivone Braga de Oliveira, pela grande força na realização deste trabalho, incentivo, companheirismo, amizade e apoio constante.