The world currently entering industry 4.0, which the industrial processes become automated and remotely control by computer with little input from human operators. Binocular vision is the result of signal sent to brain from eyes simultaneously which having advantage of depth perception. This project is about distance measurement of the object in 3-axis by using the advantages of binocular vision system. In this project, two webcams were used as binocular vision system to capture image and detect the distance measurement of the sample objects in 3-axis. The cameras must be calibrated before start to computing the distance measurement due to the fish eyes effect caused by the lens. In this project, the cameras were calibrated by using chessboard pattern with the size of 9x6 internal corners due to the simple geometry. The distance measurement algorithm will be executed once the cameras are calibrated, and the distance measurement is computed by using trigonometry concept.