The goal of this study was to investigate the effects of a standard total mixed ration (TMR) with containing anthocyanin-rich plants on animal performance, blood biochemical indices, rumen fermentation, microbial composition, and carcass characteristics in meat goats. Thirty-six healthy crossbred Thai-native Anglo-Nubian male goats (14.42 ± 0.6 kg) were used to compare the possibility of using anthocyanin-rich black cane silage (AS) in place of Napier grass silage (NS) as a functional feed resource. All goats received a 90-d routine feeding consisting of two experimental diets that were isocaloric and isonitrogenous: the control group received TMR containing 50% NS (NS; n = 18), and one group received TMR containing 50% AS (AS; n = 18). Average daily weight gain (ADG) and dry matter intake (DMI) were measured as indicators of performance. At the end of the experiment, meat, blood, and rumen samples were collected. There were no differences between the two groups in terms of final body weight, ADG, DMI, or ADG/DMI. There were no differences in rumen pH or total volatile fatty acids (VFAs); however, rumen ammonia N concentrations were lower in the AS group than in the NS group. Individual VFA concentrations varied, with AS group containing more Ruminococcus albus and NS group containing more methanogenic bacteria. Blood biochemical indices varied, with NS group having higher TBARS concentration and AS group having higher concentrations of TAC, SOD, CAT, GSH-Px, and GSH-Rx. Goat meat from the AS group had higher levels of intramuscular fat and was more tender compared to goat meat from the NS group. The feeding of anthocyanin-rich black cane appears to be an attractive alternative for Napier grass in the nutrition of meat goats. The current results indicate that the feeding of a TMR containing 50% anthocyanin-rich black cane alleviates oxidative stress and promotes the production of tender meat.

Pretreatment of lignocellulose agricultural biomass with iron prior to ensiling is required to accelerate biomass breakdown during fermentation, which could result in functional microorganisms and chemicals that reduce nutrition loss, harmful substances, and improve animal performance. The objective of this study was to investigate the effects of increasing dilutions of ferrous sulfate heptahydrate (FS) pretreatment at fresh matter concentrations of 0, 0.015, and 0.030% on the fermentation quality of black cane (BC) silage, anthocyanin stability, ruminal biogas, rumen fermentation profile, and microbial community. Pre-ensiled and silage materials were evaluated. High moisture, fiber, anthocyanin, and lignification of biomass, as well as undesirable ensiling microorganisms, were found in BC' pre-ensiled form. Increasing dilutions of FS incorporated into silages were observed to linearly decrease dry matter, anthocyanin, and nutritive value losses. The lignin values decreased linearly as the percentage of FS increased up to 0.030%. Given that the ruminants were fed pre-ensiled materials, BC silage treated with 0.030% FS dilution had comparable results to pre-ensiled BC in terms of increasing in vitro volatile fatty acid concentrations, maintaining total gas production, and reducing methane production, when compared to other FS-treated silages. In addition, BC silage treated with a 0.030% FS dilution inhibited methanogenic bacteria and regulated cellulolytic bacteria in rumen fluid. Overall, the anthocyanin content of BC remained constant throughout the rumen fermentation process after increasing dilutions of FS, indicating that BC is a viable ruminant feedstock and that pretreatment of BC with dilute FS-assisted ensiling at 0.030% could be used to generate ruminant diets.

Anthocyanin-rich black cane (aBC) is a grass rich in lignin and carbohydrates, with an abundance of anthocyanins. Silages of aBC produced with molasses (MS) and/or ferrous sulphate (FS) mixtures may have beneficial effects on silage quality and animal performance in ruminants. However, the addition of MS and FS to ensiled grass is relatively unexplored. Therefore, this study systematically evaluated the effect of their administration at different doses to select an effective treatment to modulate the ensiling characteristics of aBC. In the first trial, fresh or pre-ensiled materials (PBC) were compared with ensiled PBC treated with: 0% MS 0% FS, 4% MS, 8% MS, 0.015% FS, 0.030% FS, 4% MS + 0.015% FS, 4% MS + 0.030% FS, 8% MS + 0.015% FS, and 8% MS + 0.030% FS on a fresh matter basis. The quality of ensiling characteristics was determined in laboratory-scale silos after 42 d of preservation. Based on these results, the second trial was further conducted in rumen cultures to ensure that the selected treatment would not impair rumen fermentation. For this, ruminal biogases, rumen fermentation profiles, and microbial communities were evaluated. Ensiled PBC with the incremental addition of MS and FS resulted in the observations for anthocyanin contents and the ensiling characteristics of the aBC silages. The combination of MS (4%) and FS (0.030%) incorporated into silages had the highest silage production effect among the experimental treatments. This combination demonstrated the sustainable mitigation of the ruminal biogases of methane and carbon dioxide without impairment of total gas production. Concurrently, this combination improved total volatile fatty acid concentrations, modulated cellulolytic bacteria, and suppressed methanogenic bacteria in rumen fluids. The results presented here indicated that addition of a mixture of 4% MS and 0.030% FS to aBC resulted in an optimal balance of ensiling characteristics and is suitable for use in ruminants.