The studies on electron microstructure of the effect of the use of products that contain casein phosphopeptideamorphous calcium fluoride phosphate (CPP-ACPF) on enamel remineralization are still needed. It is important method to observe of the morphological changes of teeth in different conditions. Objective: To evaluate the remineralization potential of paste on enamel lesions using scanning electron microscopy (SEM). Methods: Sixty enamel specimens were prepared from extracted human premolars. The specimens were placed in a demineralizing solution for four days to produce artificial carious lesions. The specimens were then randomly assigned to two study groups: group A (control group) and group B. Group B was incubated in remineralizing paste (CPP-ACPF) for 30 minutes per day for 10 days. The control group received no intervention with remineralizing paste. All 60 specimens were stored in artificial saliva at 37 0 C. After remineralization, the samples were observed using SEM. Results: The statistical analysis showed a decrease in the lesion area between the demineralized and remineralized samples, but no significant difference was observed in the lesion depth for group B. There was a significant increase observed in both the lesion depth and lesion area for group A (p = 0.03). Conclusion: The results showed the capacity of CPP-ACPF in supplying calcium and phosphate to the enamel, decreasing the dissolution of the enamel surface and increasing the remineralization of the enamel surface.Keywords: CPP-ACPF, enamel, fluoride, remineralization, scanning electron microscopy How to cite this article: Ngoc VTN, Thuy PTH, Anh LQ, Son TM, Phuong NTT. The effect of casein phosphopeptideamorphous calcium fluoride phosphate on the remineralization of artificial caries lesions: an in vitro study. J Dent Indones. 2017;24(2): 45-49