The prevalence of hepatitis B virus (HBV), hepatitis C virus (HCV), and GB virus C or hepatitis G virus (GBV-C/HGV) infections was determined in 289 patients with liver disease in Ho Chi Minh City and 890 healthy inhabitants of its rural area, Dalat City, Vietnam, respectively. Serum HCV RNA and GBV-C/HGV RNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). HBsAg, HCV antibodies, and GBV-C/HGV RNA were detected in 139 (47%), 69 (23%), and ten (3%) subjects, respectively, often accompanied by elevated serum levels of alanine aminotransferase. HBsAg and HCV antibodies or HCV antibodies and GBV-C/HGV RNA were detectable simultaneously in 8% and 2% of the patients, respectively. In the inhabitants, HBsAg, HCV antibodies, and GBV-C/HGV RNA were found in 51 (5.7%), nine (1.0%), and 11 (1.2%) subjects, respectively. Thus, the prevalence of HBsAg, HCV antibodies, and GBV-C/HGV RNA was significantly higher in liver disease patients than those in the general population. In the samples from 69 patients and nine inhabitants who were seropositive for HCV antibodies, HCV RNA was detectable in 42 (61%) and 4 (44%), respectively. In patients with liver disease, ten belonged to HCV genotype 1a, ten to HCV 1b, three to HCV 2a, four to HCV 2b, and two to HCV 3a by PCR with genotype-specific primers. Nine patients had mixed genotypes, and the remaining four were not classified. Of the GBV-C/HGV RNA-positive individuals, two patients and two inhabitants were positive for HBsAg, while none of the residents had HCV antibodies, although six HCV antibodies (60%) and four HCV RNA (40%) were found in patients. When a phylogenetic tree of GBV-C/HGV was constructed based on the nucleotide sequences, the 21 isolates were classified into at least two genotypes; four isolates belonged to G2, and 17 to G3. The results indicate that in Ho Chi Minh HCV infection prevails with broad distribution of genotypes together with HBV infection among patients with liver disease. This study suggests that GBV-C/HGV infection occurs independently in the two different districts in association with HCV infection.

Among the many mutations found in the hepatitis B virus (HBV) genome, some have been associated with fulminant hepatitis, as exemplified by precore-defective mutations. The aim of this study was to determine whether such mutations also are found in Vietnamese cases of fulminant hepatitis B. The full-genome nucleotide sequence of HBV in three patients with fulminant hepatitis (F-2, F-3, and F-6) and one with acute hepatitis (A-3), who were admitted to Cho Ray Hospital, Ho Chi Minh City, Vietnam was ascertained. Additionally, two patients with fulminant hepatitis (F-1 and F-7) and three with acute hepatitis (A-1, A-2, and A-5) were examined only for the precore/core region of HBV. Remarkably, the nonsense mutation at precore codon 28 (Trp82Stop) was found in four of the five patients with fulminant hepatitis, while all the acute hepatitis patients harbored wild type (one had a mixture of wild and mutant types). The missense mutations within the core region, Ile97Leu and Pro130Ile/Thr/Ser, were also remarkable in fulminant hepatitis. Only F-2 was free from these precore/core mutations, but F-2 was unique in that it possessed a chimeric genotype: it could be classified into genotype C as a whole, but its X region was of genotype B, like the other four fulminant hepatitis isolates (F-1, F-3, F-6, and F-7). The codon 41 of the X protein was Pro in all three fulminant hepatitis cases examined for this region, while it was Ser in the wild-type isolates of genotype B. Of note as negative data, the mutations C1653T and T1753M of the enhancer II (Enh II) and A1762T and G1764A of the precore/core promoter regions, once reported to be relevant to severe or fulminant hepatitis, were not found in the present cases. The results with the Vietnamese cases of fulminant hepatitis corroborated results of previous studies with respect to the mutations Trp28Stop of precore and Ile97Leu and Pro130Ile/Thr/Ser of core, but not for the mutations within Enh II and precore/core promoter region. Whether the Ser41Pro mutation in the X region of genotype B HBV is Vietnam-specific or disease-specific deserves further investigation.

Tous droits réservés © CRIFPE, 2014Ce document est protégé par la loi sur le droit d'auteur. L'utilisation des services d'Érudit (y compris la reproduction) est assujettie à sa politique d'utilisation que vous pouvez consulter en ligne.https://apropos.erudit.org/fr/usagers/politique-dutilisation/ Cet article est diffusé et préservé par Érudit.Érudit est un consortium interuniversitaire sans but lucratif composé de l'Université de Montréal, l'Université Laval et l'Université du Québec à Montréal. Il a pour mission la promotion et la valorisation de la recherche. RésuméLes technologies de l'information et de la communication (TIC) ont pris leur essor ces derniè-res décennies. L'enseignement n'échappe pas à la tendance à introduire les TIC qui présentent des intérêts incontestables. Le présent article a pour but d'examiner la fiabilité et la validité du Modèle d'acceptation de la technologie TAM (Davis, 1989) dans un contexte vietnamien. À l'aide du questionnaire développé par Wong et Teo (2009), nous avons mené une enquête auprès de 217 étudiants vietnamiens apprenant le français langue étrangère (FLE) dans des universités où sont proposées des licences de FLE. Les données ont été traitées avec l'approche de modélisation par équations structurales. Les résultats montrent que le TAM peut être considéré comme un outil valide pour étudier les facteurs qui déterminent et qui influencent l'utilisation de la technologie dans l'apprentissage des étudiants vietnamiens. Ils indiquent également que les dimensions du TAM s'articulent entre elles de la même manière qu'ont préconisée Davis (1989. Mots-clésTIC, TAM, apprenants vietnamiens, français langue étrangère, modélisation par équations structurales Abstract Information and communication technology (ICT)has taken off in recent decades. Education is no exception to the trend of introducing ICT which presents compelling interests. This article aims to assess the reliability and the validity of the Technology Acceptance Model TAM (Davis, 1989) in a Vietnamese context. Using the questionnaire developed by Wong and Teo (2009), we conducted a survey of 217 Vietnamese students learning French as a foreign language in universities where Bachelors of Arts in French language are proposed. The data were processed with structural equation modelling approach. The results show that TAM can be considered as a valid tool to study factors which determine and influence technology use in learning by Vietnamese students. They also indicate that dimensions of TAM fit together in the same way as advocated by Davis (1989) andTeo (2009). Recherche scientifique avec données empiriques

In the context of the digital era, the role of technology in the field of tourism is vital and apparent. Technology has made traveling and tourism easier and more interesting; Internet and mobile devices have changed the way people plan and experience their trips. Tourist behavior is an underlying factor impacting the tourism services development. Understanding tourist behavior becomes prerequisite for tourism policy makers and practitioners, bringing practical values for stakeholders. Study on the impacts of technology on different stages of the travel cycle has been attracting scholars' attention. From the survey of 500 respondents living, studying or working in Ho Chi Minh City, this paper aims at mentioning the trend of applying technology in consume behavior in tourism, indicating level of technology use in different steps of the travel cycle ranging from information searching, booking, experiencing to sharing. The result showed that most of the respondents used technology at different levels in different stages of their travel cycle in which destination information searching and memories capturing were the most popular. Some implications and suggestions were also been indicated.

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