Objectives: The study aimed to describe the quality of sleep and explore factors especially Sleep Hygiene Practices associated with sleep quality among pregnant women. Study design: This is a cross-sectional study. Methods: 119 healthy pregnant women in two central hospitals in Vietnam were participated in this study. The Pittsburgh Sleep Quality Index (PSQI) was employed to evaluate sleep quality and the Modified Sleep Hygiene Practices was used to describe sleeping practices among pregnant women. Results: The results showed that while 58.8% pregnant women had quite good sleep quality, 41.2% of pregnant women had mild to moderate quality sleep disturbance. The quality of sleep was better in second trimester, but then getting worst in the third trimester. Multipara experienced worse sleep quality than nullipara. Poor sleep quality had a positive correlation with unhealthy Sleep Hygiene Practices including using an uncomfortable bed (p < 0.05), going to bed with variable bedtime (p < 0.01), watching TV or making call in bed other than sleep (p < 0.05), going to sleep without sleep sensation (p < 0.001) and staying in bed even though it was difficult to fall asleep (p < 0.001). Conclusion: Pregnant women should be taken care of on sleep quality, especially in the first and third trimester as they experience more change in hormone and physiology. Sleep Hygiene Practices should be emphasized in health education for pregnant women.