61 62In Gram-negative bacteria, porins span the outer membrane and control the influx of several prominent 63 groups of antibiotics. Thus, it should not be surprising that expression of these porins is often altered in 64 clinical isolates exhibiting multidrug resistance (MDR). The major regulator of porin expression in 65Escherichia coli is EnvZ, a canonical sensor histidine kinase (SHK). It allosterically processes 66 periplasmic interactions with MzrA and cytoplasmic osmosensing into a single unified change in the 67 ratio of its kinase and phosphatase activities. Unfortunately, the role of the EnvZ transmembrane 68 (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/206888 doi: bioRxiv preprint first posted online EnvZ regulates porin balance and antibiotic influx, these results contribute to answering the long-92 standing question of how transmembrane communication is performed by bacterial receptors. Our work 93 concludes with a framework that correlates receptor domain composition and signal transduction 94 mechanisms that could be employed by other research groups on their particular receptors of interest. 95 96 All rights reserved. No reuse allowed without permission.(which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.