Throughout the last decades, cutanous laser surgery has gained popularity among surgeons.This kind of surgery based on the principles of selective photo-thermolysis theory. CO 2 laser selectively target watercontaining tissue, applying controlled tissue vaporization. The tissue ablation and the zone of residual thermal damage is accompanied with a deeper tissue coagulation. A piece of viable intact human skin was taken. Multiple CO 2 laser incisions were carried out with different laser parameters prior to be totally excised, then a histopathological examination were done for these skin samples. Slides were examined under light microscope to evaluate thermal enjury zones, wound edges, and cutanous blood vessles status. Histopathological examination showed that the three thermal zones were chenged wherever the laser parameter changed.