Neonicotinoid insecticides
are widespread in surface waters across
the agriculturally intensive Midwestern United States. We report for
the first time the presence of three neonicotinoids in finished drinking
water and demonstrate their general persistence during conventional
water treatment. Periodic tap water grab samples were collected at
the University of Iowa over 7 weeks in 2016 (May–July) after
maize/soy planting. Clothianidin, imidacloprid, and thiamethoxam were
ubiquitously detected in finished water samples at concentrations
ranging from 0.24 to 57.3 ng/L. Samples collected along the University
of Iowa treatment train indicate no apparent removal of clothianidin
or imidacloprid, with modest thiamethoxam removal (∼50%). In
contrast, the concentrations of all neonicotinoids were substantially
lower in the Iowa City treatment facility finished water using granular
activated carbon (GAC) filtration. Batch experiments investigated
potential losses. Thiamethoxam losses are due to base-catalyzed hydrolysis
under high-pH conditions during lime softening. GAC rapidly and nearly
completely removed all three neonicotinoids. Clothianidin is susceptible
to reaction with free chlorine and may undergo at least partial transformation
during chlorination. Our work provides new insights into the persistence
of neonicotinoids and their potential for transformation during water
treatment and distribution, while also identifying GAC as a potentially
effective management tool for decreasing neonicotinoid concentrations
in finished drinking water.
The electrochemical valorization of glycerol, a by-product from biodiesel production, has received significant attention, yet systems for the efficient reforming of glycerol that are based on non-precious metals have not...
Despite its wide use as a veterinary pharmaceutical, environmental fate data is lacking for altrenogest, a potent synthetic progestin. Here, it is reported that direct photolysis of altrenogest under environmentally relevant conditions was extremely efficient and rapid (half-life ∼25 s). Photolysis rates (observed rate constant kobs = 2.7 ± 0.2 × 10(-2) s(-1)) were unaffected by changes in pH or temperature but were sensitive to oxygen concentrations (N2-saturated kobs = 9.10 ± 0.32 × 10(-2) s(-1); O2-saturated kobs = 1.38 ± 0.11 × 10(-2) s(-1)). The primary photoproduct was identified as an isomer formed via an internal 2 + 2 cycloaddition reaction; the triplet lifetime (8.4 ± 0.2 μs) and rate constant (8 × 10(4) s(-1)) of this reaction were measured using transient absorption spectroscopy. Subsequent characterization determined that this primary cycloaddition photoproduct undergoes photohydration. The resultant photostable secondary photoproducts are subject to thermal dehydration in dark conditions, leading to reversion to the primary cycloaddition photoproduct on a time scale of hours to days, with the photohydration and dehydration repeatable over several light/dark cycles. This dehydration reaction occurs more rapidly at higher temperatures and is also accelerated at both high and low pH values. In vitro androgen receptor (AR)-dependent gene transcriptional activation cell assays and in silico nuclear hormone receptor screening revealed that certain photoproducts retain significant androgenic activity, which has implications for exposure risks associated with the presence and cycling of altrenogest and its photoproducts in the environment.
Cyclotriazadisulfonamide prevents HIV entry into cells by down-modulating surface CD4 receptor expression through binding to the CD4 signal peptide. According to a two-site binding model, 28 new unsymmetrical analogues bearing a benzyl tail group and nine bearing a cyclohexylmethyl tail have been designed and synthesized. The most potent new CD4 down-modulator (40 (CK147); IC50 63 nM) has a 4-dimethylaminobenzenesulfonyl side arm. One of the two side arms was varied with substituents in different positions. This gave a range of CD4 down-modulation potencies that correlated well with anti-HIV-1 activities. The side arms of 21 of the new benzyl-tailed analogues were modeled by means of quantum mechanical calculations. For CADA analogues with arenesulfonamide side arms, the pIC50 values for CD4 down-modulation correlated with the component of the electric dipole moment in the aromatic ring, suggesting that an attractive electronic interaction is a major factor determining the stability of the complex between the molecule and its target.
I'd like to thank my advisor, David Cwiertny, for his invaluable help in completing this project. I'd also like to thank all past and present members of the Cwiertny lab-especially Nick Pflug and Monica McFadden-for their incredible help conducting experiments and analyzing data.
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