We present multi-color imaging by stimulated Raman scattering (SRS) enabled by an ultrafast fiber-based light source with integrated amplitude modulation and frame-to-frame wavelength tuning. With a relative intensity noise level of -153.7 dBc/Hz at 20.25 MHz the light source is well suited for SRS imaging and outperforms other fiber-based light source concepts for SRS imaging. The light source is tunable in under 5 ms per arbitrary wavelength step between 700 cm−1 and 3200 cm−1, which allows for addressing Raman resonances from the fingerprint to the CH-stretch region. Moreover, the compact and environmentally stable system is predestined for fast multi-color assessments of medical or rapidly evolving samples with high chemical specificity, paving the way for diagnostics and sensing outside of specialized laser laboratories.

A frequency modulation (FM) scheme for stimulated Raman scattering (SRS) is presented with a single fiber-based light source. Pulse-to-pulse wavelength-switching allows real-time subtraction of parasitic signals leaving only the resonant SRS signal with a noise reduction of up to 30 % compared to digital subtraction schemes, leading effectively to a contrast improvement by a factor of up to 8.3. The wide tuning range of the light source from 1500 cm−1 to 3000 cm−1 and the possibility to separately adjust the resonant and the nonresonant wavenumber for every specimen allow to investigate a variety of samples with high contrast and high signal-to-noise ratio, e. g., for medical diagnostics.

We present multi-color imaging by stimulated Raman scattering (SRS) enabled by an ultrafast fiber-based light source with integrated amplitude modulation and frame-to-frame wavelength tuning. With a relative intensity noise level of -153.7 dBc/Hz at 20.25 MHz the light source is well suited for SRS imaging and outperforms other fiberbased light source concepts for SRS imaging. The light source is tunable in under 5 ms per arbitrary wavelength step between 700 cm −1 and 3200 cm −1 , which allows for addressing Raman resonances from the fingerprint to the CH-stretch region. Moreover, the compact and environmentally stable system is predestined for fast multi-color assessments of medical or rapidly evolving samples with high chemical specificity, paving the way for diagnostics and sensing outside of specialized laser laboratories.

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