For the first dog, the duration of both procedures (umbilical hernia reparation and removal of the cryptorchid testicle) was 22 minutes and the skin incision was 2.0 cm length. For the second dog, procedure duration and skin incision length were 18 minutes and 2.5 cm, respectively. During the post-operative period no lesions, haemorrhages, signs of pain or other surgical complications were observed. CONCLUSIONS The criptorchidectomy performed by LESS through the commercial device is a feasible, safe and effective approach in dogs. The first case presented the ideal indication for the single port use, using the same incision for both procedures. LESS presents itself as an alternative to conventional laparoscopy in dogs with the additional advantage of only one incision, providing easy and secure organ retrieval.