In the present paper, we describe a new simple stereological method of estimating volume tensors in 3D from vertical sections. The volume tensors provide information about particle shape in 3D. In a model-based setting, the method requires that the particle distribution is invariant under rotations around the vertical axis. In a design-based approach, where the vertical section is uniformly rotated around the vertical axis, the method provides information about an index of elongation of the particles in the direction of the vertical axis. The method has been implemented on human brain tissue for the analysis of neurons in layer III of the medial frontal gyrus of Brodmann Area 46. In the actual implementation, the new estimator shows similar precision as an earlier estimator, based on an optical rotator design, but it is a factor 3 faster to collect the measurements for the new estimator. Furthermore, the calculations needed for determining the new estimator are much simpler.

Techniques involving three-dimensional (3D) tissue structure reconstruction and analysis provide a better understanding of changes in molecules and function. We have developed AutoCUTS-LM, an automated system that allows the latest advances in 3D tissue reconstruction and cellular analysis developments using light microscopy on various tissues, including archived tissue. The workflow in this paper involved advanced tissue sampling methods of the human cerebral cortex, an automated serial section collection system, digital tissue library, cell detection using convolution neural network, 3D cell reconstruction, and advanced analysis. Our results demonstrated the detailed structure of pyramidal cells (number, volume, diameter, sphericity and orientation) and their 3D spatial organization are arranged in a columnar structure. The pipeline of these combined techniques provides a detailed analysis of tissues and cells in biology and pathology.