IntroductionConsidering the increase in cardiometabolic risk after menopause, and that diet and exercise training (ET) are the pillars of prevention/treatment strategies, the objective of this study was to evaluate the metabolic, hemodynamic and autonomic effects of exercise training in an experimental model of menopausal undergoing a high‐fat diet followed by dietary adjustment. Twenty female C57BL/6J mice (8 weeks aged), all oophorectomized (bilateral ovarian removal) divided into 4 experimental groups (n = 5) were used: sedentary submitted to a high‐fat diet (OSD), sedentary submitted to 4 weeks of high‐fat diet and 4 weeks of control diet (OSRa), trained submitted to a high‐fat diet (OTD), trained submitted to 4 weeks of high‐fat diet and 4 weeks of control diet (OTRa). Oophorectomy was performed at the end of the 4th week of the protocol. Fasting glycemia and oral glucose tolerance were evaluated. ET had duration of 4 weeks (6th to 9th week of protocol, 5 days/week, 1 hour/day, intensity 50–70% of the maximum treadmill test). At the end of the protocol, the animals were cannulated for direct recording of arterial pressure (AP), after which baroreflex sensitivity and cardiovascular autonomic modulation were analyzed.RESULTSDietary adjustment promoted a reduction in glycemia and a better glucose tolerance, associated with reduction in cardiac sympathetic modulation (LF‐PI: OSD: 0.54±0.02; OSRa: 0.37±0.06; OTD: 3.0±0.02, OTRa: 0.19±0.02 ms2) and vascular (LF‐SAP: OSD: 11.3±1.7, OSRa: 3.6±0.9, OTD: 1.6±0.2; OTR:3.0±0.5mmHg2). ET, with or without dietary adjustment, increased physical capacity, reduced heart rate, improved baroreflex sensitivity, increased cardiac parasympathetic modulation (HF‐PI: OSD: 0.47±0.04, OSRa: 0,63±0.07, OTD: 0.86±0.02, OTRa: 0.75±0.07 ms2) and reduction of cardiac and vascular sympathetic modulation. However, only the association of ET with dietary adjustment was able to promote reduction in body weight, glycemia, improvement in glucose tolerance, reduction in blood pressure (mean AP: OSD: 123±2.1, OSRa: 114±0.8; OTD: 120±1.4; OTR: 110±4.0 mmHg), and further increase in parasympathetic modulation. Thus, the association of ET with dietary adjustment may be considered an important behavior for the management of cardiovascular risk in the association of obesity with ovarian deprivation.Support or Funding InformationCoordenação de Aperfeiçoamento de Pessoal de Nível Superior ‐ Brazil (CAPES) ‐ Funding Code: 001.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Despite the consensus on the benefits of moderate intensity continuous exercise for the treatment of cardiometabolic risk factors after menopause, little is known about the effects of high intensity interval training. Thus, the objective of this study was to compare the metabolic and cardiovascular effects between moderate intensity exercise training (60% of maximum exercise capacity) vs. high intensity interval training (HIIT) (90% of maximum exercise capacity, interspersed with 25% of the maximum capacity) in high‐fat diet (HF‐diet) ovariectomized mice. Twenty C57BL/6J ovariectomized mice were divided into: control (OvxC) and submitted to HF‐diet (OvxHF), HF‐diet submitted to moderate‐intensity exercise training (OvxHFT60) or submitted to HIIT (OvxHFT90). The animals were fed HF‐diet for 9 weeks, ovariectomy (bilateral surgical removal of ovaries) was performed at 5th week, and exercise training was performed from 6th to 9th weeks (4 weeks; 5 days at week; ~1hour/day). At the end of 9 weeks, blood glucose evaluations and oral glucose tolerance test were performed. Arterial pressure (AP) was assessed by direct recording (4kHz, CODAS) after 24h of carotid artery cannulation. Baroreflex sensitivity was tested by injections of phenylephrine and sodium nitroprusside. The AP records were used for analysis of cardiovascular autonomic modulation in time and frequency domains. The HF‐diet induced increase in body weight, in blood glucose, reduction in glucose tolerance, increase in mean AP (OvxC: 118±1.7; OvxHF: 123±2.1; OvxHFT60: 120±1.4; OvxHFT90: 118±2.8 mmHg) and in cardiac sympathetic modulation (LF‐PI: OvxC: 0.29±0.02; OvxHF: 0.55±0.02; OvxHFT60: 0.13±0.02; OvxHFT90: 0.33±0.09 ms2). The moderate intensity exercise training not avoid the increase in body weight, but avoid the increase in mean AP and promotes resting bradycardia, associated to baroreflex sensitivity improvement, greater cardiac parasympathetic modulation (HF‐PI: OvxC: 0.67±0.02; OvxHF: 0.47±0.04; OvxHFT60: 0.86±0.02; OvxHFT90: 0.65±0.09 ms2), and lower cardiac and vascular sympathetic modulation. The high intensity interval training promotes reduction in body weight, blood glucose (OvxC: 95±4; OvxHF: 107±2; OvxHFT60: 162±1; OvxHFT90: 134±6 mg/dL), increase in glucose tolerance, and avoid the increase in mean AP associated to baroreflex sensitivity improvements for tachycardic responses (OvxC: 4.1±1.4; OvxHF: 2.7±0.6; OvxHFT60: 8.8±0.5; OvxHF90: 7.2±0.9 bpm/mmHg). In conclusion, the high intensity interval training was more effective in promoting metabolic benefits; however, moderate intensity continuous exercise training was more effective in promoting cardiovascular benefits.Support or Funding InformationCAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior)This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.