Mitigation of banana bunchy top disease is still a challenge worldwide. BBTD is caused by banana bunchy top virus the most important virus affecting banana. Currently, no cultivar or accession has complete resistance to BBTD. A total of 36 wild Musa spp accessions including 34 Musa balbisiana and two Musa acuminata subsp. errans were screened for resistance against BBTV. In greenhouse tests using viruliferious banana aphids, all Musa balbisiana accessions remained symptomless and BBTV was not detected in any of these plants by PCR at three- and six-months post-inoculation. In contrast, 100 percent disease incidence was recorded in Musa acuminata subsp. errans and in cv. Lakatan susceptible control plants. The PCR-negative Musa balbisiana were then transferred to a field with high BBTV inoculum pressure where they remained symptomless and PCR-negative for up to five years while all cv. Lakatan developed BBTD. This study confirmed the resistance of wild Musa balbisiana accessions to BBTV. It is therefore expected to provide a resource to conventional and marker assisted breeding.