To determine the impact of a fatigue trial on the scores and number of jumps in a Tuck Jump Assessment (TJA) in female collegiate club athletes. The TJA is a 10-second plyometric jumping assessment used to replicate sport and identify lower extremity landing patterns. Scores from the TJA are summed together and those who score a 6 or higher are suggested for intervention training. Methods: Sixteen female club athletes (age: 20.9+1.9 years, weight: 59.13+7.72kg, height: 64.56+2.63cm) completed the TJA twice, once before performing a Wingate protocol and then again after. A dependent sample t-test was performed to determine any statistical differences between the TJA scores and the number of jumps between pre-Wingate and post-Wingate trials. Results: There was a statistically significant difference between scores for the TJA pre-Wingate (5.8+2.1) and post-Wingate (7.7+1.4) (P<0.0001), with nearly 2 more flaws following the Wingate protocol. There was also a statistically significant difference between the number of jumps for the pre-Wingate (15.1±3.6) and post-Wingate (16.9±2.5) (P = 0.022) with approximately 1 more jump following the Wingate protocol. Conclusions: The Wingate protocol had an impact on both the number of flaws and number of jumps, suggesting that athlete fatigue caused by completion of the Wingate protocol contributed to the increased number of errors in the TJA. The most common flaws were "pause between jumps" and "feet do not land in the same footprint". A statistically significant difference between the number of jumps pre-to post-Wingate TJA was also found. There were a greater number of jumps in the post-Wingate TJA than pre-Wingate. Due to the increased scores in the post-Wingate condition, having athletes complete a fatiguing protocol prior to the TJA may provide a more sport-like level of exhaustion. Recreating similar conditions athletes are sustaining during their sport could give a more accurate representation of the TJA, thereby making it a more clinically relevant and viable tool to use.