In 2002, apple rootstock trials using three scion cultivars were established at Geneva, NY, to evaluate 64 apple (Malus ×domestica Borkh.) rootstocks for horticultural performance and fire blight resistance. Field trials compared several elite Geneva® apple rootstocks, which were bred for tolerance to fire blight and Phytophthora root rot, to both commercial standards and elite rootstock clones from around the world. Three rootstocks performed well with all scion cultivars: ‘B.9’, ‘Geneva® 935’, and ‘Geneva® 41’. All three rootstocks were similar in size to ‘M.9’ clones but with elevated yield efficiency and superior resistance to fire blight. ‘Geneva® 11’ also performed very well with ‘Golden Delicious’ and ‘Honeycrisp’ with regard to yield efficiency and disease resistance. Resistant rootstocks greatly enhanced the survival of young trees, particularly with the susceptible scion cultivars ‘Gala’ and ‘Honeycrisp’. Results demonstrate the ability of new rootstock clones to perform better than current commercial standards, reducing financial risk to producers while promoting orchard health with enhanced disease resistance.

Streptomycin is currently the only antibiotic registered for the control of fire blight, a devastating disease of apple (Malus), pear (Pyrus), and other rosaceous plants caused by the bacterium Erwinia amylovora. Resistance of E. amylovora to streptomycin was first identified in California pear orchards in 1971 and is currently endemic in many parts of the United States. The Northeast remains the only major U.S. apple-growing region without streptomycin-resistant isolates of E. amylovora. In 2002, during a routine survey for streptomycin resistance, isolates from two neighboring orchards in Wayne County, NY were found to be highly resistant to streptomycin at a concentration of 100 μg/ml. This constitutes the first authenticated report of streptomycin resistance in New York State. Infected trees were shipped at the same time from a single nursery in Michigan. Resistance was caused by the acquisition of the strA-strB gene pair, inserted into the ubiquitous nontransmissible E. amylovora plasmid pEA29. Previously, streptomycin-resistant E. amylovora populations from Michigan were described with a similar mechanism of resistance, although the strA-strB genes are not unique to Michigan. These findings illustrate how unintentional movement of nursery material could undermine efforts to prevent the spread of antibiotic-resistant E. amylovora.

Envinia amylovora is the causative agent of apple rootstock blight. This disease is becoming more important as more susceptible and yet desirable scion cultivars are utilized in production using susceptible rootstocks. Utilization of disease resistant apple rootstocks increases the survivability of young trees infected by fire blight. Previous experiments in our breeding program showed that some apple rootstocks that derive their resistance from Mattis x rob:,sta cv. Robusta 5 show differential susceptibility to differentially virulent strains of E. anyIovora. The goal of this experiment was to expand knowledge about the inheritance of the differential susceptibility in a mapping progeny of 170 individuals of the cross between apple rootstocks Ottawa 3' and Robusta 5'. Buds from each individual in the segregating population were grafted onto seedling rootstocks and trained to a single actively growing shoot. The shoots were inoculated at the same time with a differentially virulent strain of E. wnvlovora, E2002a and a virulent strain, Fa273. The same segregating population is being used to construct a genetic map with the intention of mapping any quantitative trait loci involved in the resistance. The Geneva rootstock breeding program has developed several new rootstocks that exhibit disease resistance to E. arnylovora but a few of them have shown some susceptibility to differentially virulent strains. The results from this experiment will help us develop more durable resistance to rootstock fire blight.

Erwinia amylovora, the causal agent of fire blight, can cause a fatal infection of apple rootstocks known as rootstock blight. Budagovsky 9 (B.9) apple rootstock is reported to be highly susceptible when inoculated with E. amylovora, although results from multiple trials showed that B.9 is resistant to rootstock blight infection in field plantings. Conflicting results could stem from genetic variation in the B.9 population, appearing as phenotypic differences in rootstock material. However, genetic testing, using 23 microsatellite loci, confirmed the clonal uniformity of B.9 in commerce. Variation in growth habit between B.9 rootstocks originating from two nurseries also has been discounted as a source of disease resistance. Instead, results indicate a possible novel resistance phenotype in B.9 rootstock. B.9 rootstock was susceptible to leaf inoculation by E. amylovora, statistically similar to the susceptible rootstock Malling 9 (M.9). Conversely, inoculation assays targeting woody 4- to 5-year-old tissue revealed a high level of resistance in B.9, whereas M.9 remained susceptible. Although the mechanism by which B.9 gains resistance to E. amylovora is unknown, it is reminiscent of age-related resistance, due to an observed gain of resistance in woody rootstock tissue over succulent shoot tissue. Durable fire blight resistance correlated with tissue development could be a valuable tool for rootstock breeders.

No abstract