A simple, fast and sensitive high-performance liquid chromatography/electrospray ionization-mass spectrometry method is described and validated in terms of linearity, accuracy/recovery and reproducibility, as well as limit of detection for the determination of ascorbic acid from grape (Vitis vinifera L.) samples from Murfatlar vineyard. Good linearity (correlation factor > 0.9970) was achieved in the concentration range 0.5-15 mg mL À1 for AA in acetic acid 0.1% solution. The obtained RSD values (below 5%) indicated excellent repeatability of the proposed method. The limit of detection was 0.32 mg mL À1 while the recovery ranged between 96.86 and 102.41%. The advantages of the method are: small amounts of sample and solvents, short analysis time and minimum steps for sample preparation.

The objective of the present work was to adapt the Prussian Blue reaction for the determination of ascorbic acid. The procedure was successfully applied for the determination of ascorbic acid in red and white grapes (Vitis vinifera L.) just previous ingathering. In the present work was used the red and white grapes from Murfatlar vineyard: Mamaia, Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, Sauvignon, Muscat Ottonel and Riesling Italian. The results were situated in the range of 0.67 -1.79 mg vitamin C/100g product for red grapes and respectively 0.50 -1.49 mg vitamin C/100g for white grapes.

The study investigates the pollution with heavy metals of grapes and soil. The grapes nourish from the respective soil, with all existing substances: either nutrients or toxic materials. This link, between grapes and soil, made mandatory to focus on observing the level of toxic materials in both samples grapes and land. The aim of this research is to analyze the level of Cd and Pb in Vitis vinifera L. grape fruits and soil, by flame atomic absorption spectrometry (FAAS) method. The grapes and the soil used in this work were sampled from the Murfatlar City, a nonindustrial area, placed far from the car traffic pollution. Cd and Pb were quantified, after the chemical mineralization of the samples using nitric acid. It can be noticed that the values of cadmium and lead concentrations in grapes were lower than the recommendable maximum limit.

Abstract. The aim of research is to assess the active substance by a HPLC method for the separation and quantitative determination of loratadine. The method has been developed and validated on the standard solutions, in previous research. The current study was undertaken to present the results obtained from loratadine determination in biological samples (human serum, urine and breast milk). These results may be applicable on patients with different physiological conditions (aging, pregnancy or recently giving birth, etc.) and pathological conditions which may interfere with the metabolism of loratadine. The used HPLC method detected loratadine concentrations in human serum samples, respectively urine samples, at 2 hours after drug administration. The method detected traces of loratadine which passed into breast milk, as well. Data were statistically interpreted using MED CALC 10.2 software. These results show that the applied method can be used for quantitative analysis of loratadine in biological fluids (all permissible limits of quality specifications being in the range 95-105%).

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