Pangolins are among the most extensively traded taxa in southeastern Asia mainly due to the perceived medicinal value of their scales and other body parts, putting them at risk of extinction, however, little is known about their trade status in Nepal. The purpose of the study was to assess the status of pangolin trade in Makwanpur district of Nepal. Semi-structured interview with household (n = 90), key informant survey (n = 15), Four focus group discussion at each study site was conducted. Seizure data (2015–2019) were gathered from the law enforcement agencies to identify and analyze the major trade routes. The majority of the respondents (63%) were well aware of the protection status of pangolins. Further, our study found that historically local peoples used pangolin body parts in making rings, bags, jackets, and musical instruments but at present they stopped it. Pangolin traders were typically middle-aged men and unemployed youth. Majority of the hunting was found to be opportunistic but when pangolins were caught, they were generally sold for additional income. Currently, the seizure data has shown the declining trend of pangolin trade within the Makwanpur district. Community forest user groups and community based anti-poaching units are working actively for the conservation and promulgation of threatened pangolins in the Makwanpur district which had long served as a major trade route to China. Thus, we advocate strengthening border security and the formation of community-based anti-poaching units, followed by mobilization, anti-poaching trainings, security assurance, and incentives for worthy conservation results in pangolin-rich communities. Further, we recommend sustained conservation awareness programs, in addition to alternative livelihood opportunities, for the long-term conservation of pangolins and their habitat.