Understanding water-soluble and environmentally friendly two-photon absorption (TPA) molecules benefits the design of superior organic complexes for biomedical, illumination, and display applications. In this work, we designed two triphenylamine-based all-organic compounds and explored the mechanism of enhanced TP fluorescence in water solutions for potential applications. Experimentally, we showed that adding protein into our TPA molecule solution can drastically boost the TP fluorescence. Numerical simulations reveal that the TPA molecules prefer to dock inside the protein complex. We hypothesize that the interaction between our triphenylamine-based all-organic compounds and water molecules lead to non-radiative decay processes, which prevent strong TP fluorescence in the water solution. Therefore, the protection by, for example, protein molecules from such interactions can be a universal strategy for superior functioning of organic TPA molecules. Further experiments and numerical simulations support our hypothesis. The present study may facilitate the design of superior water-soluble and environmentally friendly superior organic complexes.

The mycobacteriophages JeTaime (E cluster) and Luna22 (Q cluster) were isolated from soil in Providence, Rhode Island, and Charleston, South Carolina, respectively, using a Mycobacterium smegmatis mc 2 155 host. The genome of JeTaime is 75,099 bp (142 predicted genes), and that of Luna22 is 53,730 bp (87 predicted genes). Both phages exhibit Siphoviridae morphology.