Industrial tomatoes are cultivated in about 4000 ha of the Pinios river basin (central Greece), providing significant income to the farmers. In this study, the water footprint (WF) of industrial tomatoes between planting and harvest was estimated in 24 different farms for three consecutive years. The selected farms were representative of the main agro-climatic zones and soil textural classes within the river basin. Green, blue and grey WF calculations were based on datasets of the experimental plots for each farm, including irrigation water volume, meteorological, soil, and crop yield data. The results showed that the WF of tomatoes ranged from 37 to 131 m 3 water/ton tomatoes with an average of 61 m 3 /ton. The WF variation depended mainly on crop yield, local agro-climatic and soil conditions. The green, blue, and grey WF components averaged 13, 27 and 21 m 3 /ton, respectively. The results reveal the importance of WF in understanding how tomato production relates to the sustainable use of freshwater and pollution at local level.